呼吸道合胞病毒感染相关性哮喘的发病机制研究  

Pathogenesis of respiratory syncytial virus infections-related asthma

作　　者：殷晓霞[1] 温翠玲[2] 刘建华[2] 张斌[3] 

机构地区：[1]河北北方学院研究生院,河北张家口075000 [2]河北北方学院附属第一医院呼吸科,河北张家口075000 [3]河北北方学院附属第一医院检验科,河北张家口075000

出　　处：《中华医院感染学杂志》2015年第9期1940-1942,共3页Chinese Journal of Nosocomiology

基　　金：河北省科技厅科研基金资助项目(DC000671)

摘　　要：目的通过研究神经生长因子(NGF)与血清肾上腺素浓度之间的关系,探讨呼吸道合胞病毒(RSV)感染相关性哮喘的发病机制,指导临床诊治RSV感染相关性哮喘。方法取健康的SD幼鼠60只随机分为3组,分别为RSV组、抗NGF组及对照组,每组20只,SD幼鼠培养3个月后处死,取肾上腺组织、血清等,取SD大鼠肾上腺组织,利用电子显微镜、试剂盒、免疫组化ELISA法及RT-PCR检测观察肾上腺髓质细胞的超微结构变化、NGF和肾上腺素浓度及NGF与神经丝蛋白的表达量。结果 SD大鼠肾上腺髓质细胞NGF的表达量RSV组明显高于抗NGF组和对照组、抗NGF组S明显高于对照组,差异有统计学意义(P<0.05);SD大鼠血清中NGF浓度RSV组明显高于对照组和抗NGF组,差异有统计学意义(P<0.05);SD大鼠血清中肾上腺素的浓度RSV组和抗NGF组明显低于对照组、RSV组明显弱于抗NGF组,差异有统计学意义(P<0.05);SD大鼠肾上腺髓质细胞神经丝mRNA的表达量RSV组和抗NGF组较对照组明显升高、RSV组较抗NGF组SD大鼠明显升高,差异有统计学意义(P<0.05)。结论 RSV感染相关性哮喘的发病机制可能为其感染后造成肾上腺组织和血清NGF的表达量升高,NGF促进肾上腺髓质细胞向神经元转化,导致血清中肾上腺素浓度降低,不能维持支气管的舒张,从而引起哮喘的发生。OBJECTIVE To study the relationship between the nerve growth factor （NGF） and the concentration of serum adrenaline and explore the pathogenesis of respiratory syncytial virus （RSV） infection‐related asthma so as to provide guidance for clinical diagnosis and treatment of the RSV infection‐related asthma .METHODS A total of 60 healthy SD rats were randomly divided into three groups ：the RSV group ,the anti‐NGF group ,and the control group ,with 20 cases in each .The SD rats were killed after the culture for three months ,then the adrenal tissue and serum were collected;the change of the ultramicro‐structure of adrenal medullary cells was observed by using electron microscope ,the concentration of NGF was determined with the use of ELISA ,the concentration of serum adrenaline was tested ,and the expression of NF‐L mRNA was determined by means of RT‐PCR .RESULTS The NGF expression of the RSV group was significantly higher than that of the anti‐NGF group and the control group , and the NGF expression of the anti‐NGF group was significantly higher than that of the control group（P〈0 .05） . The concentration of serum NGF of the RSV group was significantly higher than that of the control group and the anti‐NGF group（P〈0 .05） .the concentration of serum adrenaline of the RSV group and the anti‐NGF group was significantly lower than that of the control group ,while the concentration of serum adrenaline of the RSV group was significantly lower than that of the anti‐NGF group（P〈0 .05） .The expression volume of medullary cells NF‐L mRNA of the RSV group and the anti‐NGF group were significantly higher than that of the control group ,while the expression volume of medullary cells NF‐L mRNA of the RSV group was significantly higher than that of the anti‐NGF group（P〈0 .05） .CONCLUSION The pathogenesis of the RSV infection‐related asthma may lead to the elevated expression volume of adrenal tissue and serum NGF ,the NGF promotes the transformation of adrenal me‐

关 键 词：呼吸道合胞病毒 哮喘 发病机制 

分 类 号：R373[医药卫生—病原生物学]