快速测定铁皮石斛总生物碱含量方法的优化  

Improvement of Rapid Method to Determine Total Alkaloids Contents of Dendrobium officinale

作　　者：刘莉[1,2] 钱均祥[1] 萧凤回[2] 查应洪 段承俐[2] 杨生超[2] 肖丹[1] 吴丽华[1] 李智敏[1] 张庭[1] 潘俊[1] 王翰墨[1] 李晚谊[1] 

机构地区：[1]云南省农业科学院药用植物研究所,云南昆明650223 [2]云南农业大学云南省中药材规范化种植指导中心,云南昆明650201 [3]云南群鑫石斛种植有限公司,云南屏边661200

出　　处：《西南农业学报》2015年第2期575-579,共5页Southwest China Journal of Agricultural Sciences

基　　金：云南省自然科学基金项目(2012FB193)

摘　　要：通过取样量、超声时间、浸提剂量、粒径等4个石斛生物碱的超声提取影响因素的正交和单因素试验。结果表明,石斛生物碱含量超声提取测定的最佳参数:0.050 g的取样量、60目的粒径、50 min的超声时间、60的浸提试剂用量与取样量比。该方法具有样品前期处理时间短,测定仪器操作简便,测定速度快、样品用量少的特点,能在较短时间内对大量单株个体进行检测鉴定,为石斛的品质育种提供了有效途径和手段。Four factors influencing the extract by ultrasonic,namely sampling quantity,treating time,volume of extracting solution and granule size were researched synthetically on the basis of orthogonal experiment and one-factor tests. The results showed that the best parameters for the extraction of the total alkaloids content of Dendrobium was determined as follows： sampling quantity was 0. 050 g crushed to 60 eye and treating time was 50 min and the ratio of power to time for the sample-treating in advance,simple operation and rapidity. Above all,the volume of extracting solution was 1∶ 60. The advantages of methods including short method would be applied to determine total alkaloids content of plentiful plants in a short time and the genotype individual possessing of high content of alkaloids might be screened out,which can be used as the effective measure in the quality-meliorating of Dendrobium.

关 键 词：石斛 超声提取 总生物碱含量 

分 类 号：S567.9[农业科学—中草药栽培]