Dynamic changes of interleukin-1, interleukin-6 and tumor necrosis factor in intermingled skin graft in burned rats  

作　　者：吴建和 赵雁飞 胡宝瑜 童善庆 朱德安 陆德源 史济湘 

机构地区：[1]DepartmentofMicrobiology,ShanghaiSecondMedicalUniversity [2]DepartmentofPathology,ShanghaiSecondMedicalUniversity [3]InstituteofBurnResearch,RuijinHospital,ShanghaiSecondMedicalUniversity

出　　处：《Chinese Journal of Traumatology》2001年第1期31-36,共6页中华创伤杂志（英文版）

基　　金：This work was supported by the National Natural ScienceFoundation of China!(No .392 90 70 0 6 )

摘　　要：To compare the dynamic changes of interleukin 1 (IL 1), interleukin 6 (IL 6), and tumor necrosis factor (TNF) in intermingled skin graft with those in other types of skin grafts in rats. Methods: A 10% 15% third degree burn was created in 180 Spregue Dawley (SD) rats. After removing the scar, skin grafts were performed on the open wounds immediately with autoskin (aus, n=54), alloskin (als, n=54) and intermingled skin (n=36). That is to say, in the intermingled skin graft, a big piece of alloskin (mals) was grafted first, and 3 days later, small pieces of autoskin (maus) were embedded in the alloskin. The rest 36 rats were taken as the controls. And the biological activities of IL 1, IL 6 and TNF in graft sheets in each group were detected after skin graft. Results: The levels of IL 1, IL 6 and TNF in the aus group decreased steadily after their initial elevations, whereas in the als group they increased significantly and kept on the peak level in the later phases. In the intermingled group, there appeared a lowest IL 1 level in the mals and a highest one in the maus simultaneously at 7 (4) days (The number out of parenthesis is the days after transplanting with alloskin sheets, and the number in parenthesis is the days after embedding autoskin sheets in the intermingled skin graft. Similarly hereinafter.) after skin graft (P< 0.01 ), and the high level in the maus abruptly decreased at 14 (11) days after skin graft. At exactly the same phase on day 7 (4), a prominent peaked IL 6 in the mals occurred. In the later phases, the levels of TNF remained relatively low both in the mals and in the maus. From day 7 (4) on, each cytokine fluctuation in the mals synchronized with that in the maus. The longer the post transplantation period lasted, the more the positive cytokine correlated between the mals and the maus. Conclusions: The low levels of IL 1 and TNF may be important factors to lighten the intensity of local rejection in the intermingled skin graft. The temporarily peaked IL 6 is both an inducer which induces t

关 键 词：Skin transplantation BURNS Interleukin  1 Interleukin  6 Tumor necrosis factor 

分 类 号：R644[医药卫生—外科学]