山西大豆自然群体遗传多样性的研究  

Research on Genetic Diversity of Natural Population of Soybeans in Shanxi Province

作　　者：郭数进[1] 杨凯敏[1] 霍瑾[1] 周永航[1] 王宏勇[1] 李贵全[1] 

机构地区：[1]山西农业大学农学院,山西太谷030801

出　　处：《天津农业科学》2015年第5期1-6,共6页Tianjin Agricultural Sciences

基　　金：山西省科技攻关项目(20120311005-3);山西省农业科技成果转化资金项目;山西农业大学科技创新基金项目(育种基金)(2006057)

摘　　要：为探明由山西不同生态型大豆品种组成的自然群体的遗传多样性,为优异种质资源的筛选和应用提供理论依据,以102个大豆品种组成的自然群体为研究材料,采用59对SSR引物对该群体进行遗传多样性分析。结果表明：59对引物最终筛选出50对多态性好的引物,共检测出157个等位变异,不同位点的等位变异数为2~7个,平均等位变异数为3.14个,其中等位变异数最多的为Satt263,有7个。香农指数变幅为0.097 3~1.668 4,香农指数平均值为0.844 9,多态性信息量的变幅为0.038~0.761,平均每个引物的多态性信息量为0.431。标记将102个大豆材料在遗传距离GD=0.805处聚为3个群组,群组1有52个材料,群组2由48个材料组成,群组3仅有2个材料。In order to probe genetic diversity of a natural population composed of different eco-types of soybeans and provide theoretic foundation for screening and applying excellent germplasm, a natural population including 102 soybean cultivars randomly was used as material tested and 59 pairs of SSR primers were employed to analyze genetic diversity of the population. The results showed that 50 pairs of primers with outstanding polymorphism were screened from the 59 pairs of primers. 157 allelic variations were detected, the numbers of allelic variation at different sites were 2-7, the average variation was 3.14, and the primer Satt263 had 7 variations which was the most. The changing range of Shannon index was 0.097 3-1.668 4, its average value was 0.844 9. The changing range of polymorphism information was 0.038-0.761, and polymorphism of each primer was averagely 0.431. The markers classified the cuhivars into 3 groups at GD=0.805, Groupl included 52 cuhivars, Group2 had 48 cultivars, and Group3 only possessed 2 euhivars.

关 键 词：大豆 自然群体 分子标记 遗传多样性 

分 类 号：S565.1[农业科学—作物学]