参附配伍减毒作用的尿液代谢组学研究  

Urine metabolomics studies of Shenfu( 参附) attenuated compatility

作　　者：赵佳伟[1,2] 何家乐[1,2] 马增春[1] 梁乾德[1] 王宇光[1] 谭洪玲[1] 肖成荣[1] 汤响林[1] 高月[1] 

机构地区：[1]军事医学科学院放射与辐射医学研究所,北京100850 [2]安徽医科大学研究生学院,合肥230032

出　　处：《中药药理与临床》2015年第1期1-5,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家重点基础研究发展计划("973计划")(2011CB505304;2012CB518402);国家自然基金(81274127)

摘　　要：目的:研究参附配伍对大鼠尿液代谢组学的影响,旨在从尿液中发现参附配伍作用的内源性标记物,从内源性代谢物角度解释参附配伍的作用规律。方法:连续7天给予雄性Wistar大鼠不同配伍的参附水煎液20g/kg,收集尿液,通过主成分分析和偏最小二乘判别法分析参附配伍对内源性代谢物的影响。结果:参附合并组与合煎组相比,泛醌、马尿酸的含量下降,甲硫氨酸含量上升。泛醌、泛酸、核黄素、甲硫氨酸等可能通过抗氧化的方式来减轻附子的毒性,并影响着线粒体的功能。结论:参附配伍可以减轻附子的毒性,并且代谢组学为参附配伍减毒的研究提供了科学参考。Objective： Research the effect of Shenfu formulation of cardiac toxicity in rats,Explore the relationship between cardiovascular disease between endogenous marker. Methods： Different compatibility of Shenfu decoction were given to male Wistar rats at a dosage of 20 g / kg for7 days,collected the urine,and analysis the endogenous metabolites effected by Shenfu formulation by principal component analysis and partial least-squares analysis. Result： Shenfu co-decoction could alleviated Fuzi＇s toxicity effectively compared with mixed-decoction,the contents of Ubiquinone Q2 and Hippuric acid decreased while L-Methionine increased. Ubiquinone Q2,Pantothenic acid,Riboflavin,L-Methionine could alleviated Fuzi＇s toxicity by antioxidant and affected function of mitochondria. Conclusion： Shenfu compatibility can reduce cardiac toxicity induced bu fuzi,Metabolomics provided a scientific reference of compatibility attenuate of Shenfu.

关 键 词：人参 附子 配伍减毒 内源性标示物 尿液 代谢组学 

分 类 号：R285.5[医药卫生—中药学]