小半夏汤对化疗性异食癖大鼠P物质和NK1受体的影响  

Effect of Xiaobanxiatang on substance P and NK1 receptor in chemotherapy-induced pica in rats

作　　者：于功昌[1,2] 张勇[1] 杜秀伟[1] 聂克[3] 

机构地区：[1]山东中医药大学,济南250355 [2]山东省职业卫生与职业病防治研究院,济南250062 [3]山东中医药大学基础医学院,济南250355

出　　处：《中药药理与临床》2015年第1期17-20,共4页Pharmacology and Clinics of Chinese Materia Medica

基　　金：国家自然科学基金面上项目(编号:81373828);高等学校博士学科点专项科研基金联合资助课题(博导类)(编号:20123731110004);山东省自然科学基金项目(编号:ZR2012HM058)

摘　　要：目的:观察小半夏汤对化疗性异食癖大鼠P物质和NK1受体的影响,探讨小半夏汤防治化疗性恶心呕吐的作用机制。方法:ip注射6mg/kg顺铂建立大鼠化疗性异食癖模型。将大鼠随机分为正常对照组、小半夏汤对照组1.6g/kg、顺铂模型组、昂丹司琼阳性药组2.6mg/kg、小半夏汤大剂量组3.2g/kg和小半夏汤小剂量组1.6g/kg。各组动物在造模前1 h首次灌胃给药,之后每12h给药一次,正常对照组和模型组灌胃等容积蒸馏水。每12h称量并计算各组动物高岭土摄入量;分别于造模后24h和72h处理动物,采用CLIA和ELISA法测定大鼠血清、回肠和延髓P物质含量,采用Real-time PCR法测定大鼠回肠和延髓前速激肽原A(PPTA)和NK1受体mRNA表达。结果:顺铂模型组动物摄食高岭土量显著升高,3.2、1.6g/kg小半夏汤均可抑制化疗大鼠摄食高岭土量,降低化疗大鼠血清、回肠和延髓P物质含量,抑制回肠和延髓PPTA和NK1受体mRNA的异常表达。结论:小半夏汤防治化疗性恶心呕吐部分作用机制可能与降低P物质含量、下调NK1受体mRNA表达有关。Objective： In order to explore the mechanism of Xiaobanxiatang（ XBXT） on the prevention and treatment of chemotherapy-induced nausea and vomiting（ CINV）,the effect of XBXT on substance P and NK1 receptor in chemotherapy-induced pica in rats was observed.Methods： The chemotherapy rat pica model was established by ip injection of 6mg / kg cisplatin. The Wistar rats were randomly divided into normal control group,XBXT control group（ 1. 6g / kg）,cisplatin model group,positive drug ondansetron group（ 2. 6mg / kg）,XBXT high（ 3. 2g/kg） and low（ 1. 6g/kg） dose groups. The rats in ondansetron group,XBXT control group,XBXT high and low dose groups were respectively given 2. 6mg / kg / d ondansetron,1. 6,3. 2 and 1. 6g / kg / d XBXT by oral gavage before modeling 1h and then twice a day. The rats in normal control group and model group were gavaged with equal volume of distilled water. Kaolin consumptions were weighed and calculated once per 12 h. After modeling 24 h and 72 h,substance P contents in rat serum,ileum and medulla oblongata were measured by the methods of CLIA and ELISA,and preprotachykinin A（ PPTA） and NK1 receptor mRNA expression levels in ileum and medulla oblongata were measured by Real-time PCR. Results： Kaolin consumptions in model group were significantly increased. 3. 2 and 1. 6g / kg XBXT could significantly inhibit kaolin consumptions in cisplatin-treated rats,reduce substance P contents in serum,ileum and medulla oblongata,and inhibit the abnormal expression of PPTA and NK1 receptor mRNA expression in ileum and medulla oblongata. Conclusion： The mechanism of XBXT on the prevention and treatment of CINV was related to the decrease of substance P contents and down regulation of NK1 receptor mRNA expression induced by cisplatin.

关 键 词：小半夏汤 异食癖大鼠 化疗性恶心呕吐 P物质 NK1受体 

分 类 号：R285.5[医药卫生—中药学]