威灵仙总皂苷对佐剂性关节炎(AA)大鼠IL-6、IL-10及滑膜中p-JAK2、p-STAT3表达的影响  

Effects of total saponins of Clematis on the levels of IL-6,IL-10 and the expression of p-JAK2,p-STAT3 in adjuvant-induced arthritis rats

作　　者：汪永忠[1] 邓龙飞[2] 韩燕全[1] 姜辉[1] 李钰馨 

机构地区：[1]安徽中医药大学第一附属医院、国家中医药管理局中药制剂三级实验室,合肥230031 [2]安徽中医药大学,合肥230038

出　　处：《中药药理与临床》2015年第1期86-90,共5页Pharmacology and Clinics of Chinese Materia Medica

基　　金：安徽省人事厅人才基金项目(NO.2005Z035);安徽省自然科学基金项目(1508085MH201);国家中医药重点学科临床中药学建设项目(国中医药人教发[2012]32号)

摘　　要：目的:研究威灵仙总皂苷(TSC)对佐剂性关节炎(AA)大鼠细胞因子白细胞介素-6(interleukin-6,IL-6)、白细胞介素-10(interleukin-10,IL-10)及滑膜组织中磷酸化Janus激酶2(phospho-JAK2,p-JAK2)、磷酸化信号转导子和转录激活子3(phospho-STAT3,p-STAT3)蛋白表达的影响。方法:雄性大鼠60只随机分为6组,除正常组外,采用弗氏完全佐剂诱导AA大鼠模型。致炎第12天后灌胃给予相应药物,每天1次,连续16天,观察药物对AA大鼠体重影响;对AA大鼠进行关节指数评分;HE染色固定部位踝关节进行病理学观察;酶联免疫吸附实验(ELISA)法测血清中IL-6、IL-10的含量;免疫组化测滑膜组织中p-JAK2、p-STAT3蛋白表达。结果:与模型组相比,威灵仙总皂苷(50、100、200mg/kg)组均可抑制AA大鼠足肿胀,减轻踝关节病理损伤,其中威灵仙总皂苷(100、200mg/kg)组治疗结果具有统计学意义;威灵仙总皂苷(100、200mg/kg)可有效抑制血清中IL-6及提高IL-10的含量;威灵仙总皂苷(50、100、200mg/kg)给药干预后均可抑制p-JAK2、p-STAT3蛋白的阳性表达。结论:威灵仙总皂苷对AA大鼠具有较好的治疗效果,其机制可能与抑制IL-6和提高IL-10的含量,抑制p-JAK2、p-STAT3蛋白的表达,从而调控JAK2/STAT3通路有关。Objective： To evaluate the effect of total saponins of Clematis（ TSC） not only on the levels of IL-6,IL-10,but also on the expression of phosphor-Janus kinase 2 and activator of phosphor-transcription 3 in adjuvant-induced arthritis（ AA） rats. Methods： Sixty SD rats were randomized into six groups： normal group,model control group,TSC（ 50,100,200 mg / kg） group,and tripterygium glycosides tablet（ 10 mg / kg）group. In addition to the normal group,AA was induced with Freund＇s complete adjuvant. The treated groups orally took TSC with different dose and tripterygium glycosides tablet after 12 days,q. d for 16 days. Then,the effects of drugs on the body weight and score the ankle-joint index were observerd,ankle-joint samples were taken to examine the degree of AA by HE staining. ELISA was used to detect IL-6 and IL-10 levels. Moreover,the expressions of p-JAK2 and p-STAT3 protein were detected by immunohistochemistry. Results： Compared with the model group,TSC（ 50,100,200 mg / kg） group could effectively inhibit AA rat paw swelling,reduce pathological ankle injuried,in Which TSC（ 100,200mg/kg） group was statistically significant results（ P 〈0. 05）. Meanwhile inhibited the level of IL-6 and improve the level of IL-10 in serum by TSC（ 100,200 mg / kg） group（ P 〈0. 05）. Furthermore,treatment with TSC（ 50,100,200 mg / kg） could decrease the expression of p-JAK2 and p-STAT3 protein from synovial tissues. Conclusion： TSC has a better therapeutic effect on AA rats,the mechanism may be related to inhibited the level of IL-6 and improved the level of IL-10,as well as inhibited the expression of p-JAK2,p-STAT3 protein,regulated JAK2 / STAT3 signal pathway to achieve anti-inflammatory effects.

关 键 词：威灵仙总皂苷 佐剂性关节炎 免疫组化 

分 类 号：R285.5[医药卫生—中药学]