基于线粒体DNA序列的秦岭地区淡足青步甲种群遗传结构分析  

Population genetic structure of the ground beetle Chlaenius pallipes from the Tsinling Mountains based on mitochondrial DNA analysis

作　　者：阴环[1] 李晓晨[2] 

机构地区：[1]山西师范大学生命科学学院,临汾041004 [2]陕西师范大学生命科学学院,西安710062

出　　处：《生态学报》2015年第9期3052-3059,共8页Acta Ecologica Sinica

基　　金：山西省青年科技研究基金项目(2012021026-1)

摘　　要：淡足青步甲Chlaenius pallipes是一类重要的天敌昆虫。为了揭示秦岭地区淡足青步甲的种群遗传分化和种群历史动态,对13个地理种群151个个体的线粒体Cox1-tRNALeu-Cox2片段DNA序列进行了分析。序列比对后的全长为1 602 bp,共检测到57个多态性位点,定义65个单倍型,其中48个为居群内特有单倍型,17个为居群间共享单倍型。单倍型多样性指数Hd为0.972,核苷酸多样性指数Pi为0.0025。该种群遗传分化明显,但AMOVA分析结果表明变异主要来源于种群内,占变异总量的92.10%。SAMOVA和PERMUT分析结果均表明秦岭地区的淡足青步甲种群不存在明显的谱系地理结构。结合中性检验、错配分布和BSP分析结果表明该物种发生过种群扩张,且扩张时间大致在0.100—0.025 Ma之间。The ground beetle,Chlaenius pallipes,belonging to the genus Chlaeniu（ Coleoptera： Carabidae）,is widely distributed in East Asia and overwinters as adults. It is highly abundant in northern China and plays an important role in agriculture and forest pest control. To understand the population genetic differentiation and demographic history of C.pallipes from the Tsinling Mountains,a 1,602 bp fragment of mitochondrial DNA（ Cox1-tRNALeu-Cox2） was sequenced for151 individuals from 13 geographical populations from Gansu,Shaanxi,and Henan provinces. Fifty-seven polymorphic sites were identified, of which 45 are parsimony informative,11 singleton variable sites, and a 1 bp insertion. These polymorphic sites defined 65 haplotypes, among which 17 were common and 48 were private with relatively lower frequencies. The haplotype diversity（ Hd= 0. 972） was hight but the nucleotide diversity was low（ Pi= 0. 0025）. The topologies of the phylogenetic trees obtained based on maximum likelihood（ ML） and Bayesian inference were similar. Both ML tree and Bayesian inference tree revealed two clades（ a and b） from node d on the trees. In the haplotype network,a five median vector was employed and several loops were constructed and＂star phylogeny＂patterns were present. Analysis of molecular variance（ AMOVA） suggested that most of the variation was due to within-population differences（ 92. 10%）,while differences among populations only contributed 7. 90% of the total. The among-population FSTvalue was 0. 07897（ P0. 01）. Consequently,we conclude that there is obvious genetic differentiation in the C. pallipes populations we investigated. For the spatial analysis of molecular variance（ SAMOVA）,increasing K values from 2 to 12 were selected;the FCTvalues were fairly low and fluctuated. Thus,the SAMOVA tests failed to reveal any meaningful phylogeographic structure. Furthermore,the results of the PERMUT analysis did not suggest any phylogeographic structure of the populations sampled based on the

关 键 词：淡足青步甲 线粒体DNA 种群结构 数量扩张 秦岭 

分 类 号：S476[农业科学—农业昆虫与害虫防治]