缺血预处理对大鼠肝脏缺血再灌注损伤后细胞凋亡的影响  

Effects of ischemic preconditioning on apoptosis after ischemia-reperfusion liver injury in rats

作　　者：戴勇[1] 陶立德[1] 薛同敏[1] 张培建[1] 刘霞[1] 朱以佳[1] 陈国凤[1] 

机构地区：[1]扬州大学第二临床医学院普通外科研究室,江苏扬州215000

出　　处：《中国现代普通外科进展》2015年第3期169-172,共4页Chinese Journal of Current Advances in General Surgery

基　　金：江苏省扬州市社会发展科技攻关项目基金资助项目(YZ2014064)

摘　　要：目的:研究缺血预处理(IP)对大鼠肝脏缺血再灌注(IR)损伤后细胞凋亡的影响。方法 :健康成年雄性SD大鼠随机分为3组(每组5只)。SO组(假手术组):只麻醉开腹,不阻断肝脏血流;IR组:采用Pringle法大鼠肝脏缺血30m in再灌注3 h;IP组:在IR前先阻断肝门血流10 m in,然后开放血流10m in,其余步骤同IR组。各组均在术后3h后取组织进行检测。流式细胞术检测细胞凋亡;W-B法检测凋亡相关蛋白B cl-2、C aspase-3;生化分析仪检测A ST、A LT的活性。结果:与单纯IR相比,IP预处理明显降低了再灌注3 h所致肝组织细胞凋亡率(P<0.05);同时增加肝组织抗凋亡蛋白B cl-2含量,降低凋亡蛋白C aspase-3的表达(P<0.05);降低血中A ST、A LT的活性(P<0.05)。结论:IP处理减少肝脏IR期间的细胞凋亡比率,其主要机制可能通过增加抗凋亡蛋白B cl-2表达,降低凋亡蛋白C aspase-3表达来实现,同时伴随血清A ST、A LT减少,从而减轻IR术后肝功能的损害。Objective： To investigate the effects of ischemic preconditioning（IP）on apoptosis after is chemia-reperfusion（IR） injury.Methods： U sing rat model of intestine IR injury,Sprague-D awley male rats were divided into 3 groups with 5 in each group： shamoperation（SO）group, I/R group（using Pringle method hepatic ischemia 30 min followeclby reperfusion 3 h）and IP（B efore I/R to block thehepatic portal blood flow10 min, and then open the bloodstream10 min）group,respectively. Flowcytometry to detect cell apoptosis, The activity of serumalanine aminotransferase（ALT）and aspartate aminotransferase（AST）were determined and the expressions of B cl-2 and C aspase-3 in each group. Result： C ompared with that in I/R group, IP significantly reduced cell apoptosis rate after 3h later in liver tissue（P〈0.05）, while increasing B cl-2 and reducing C aspase-3 expression（P〈0.05）, and also reduce AST, ALT activity（P〈0.05）. Conclusion： IP processing to reduce the rate of apoptosis in IR period, the main mechanismmay B cl-2 protein expression and reduced C aspase-3 expression to achieve, accompanied by AST, ALT decreased,thereby reducing damage to liver function after IR postoperative.

关 键 词：肝脏缺血再灌注 缺血预处理 凋亡 

分 类 号：R657.3[医药卫生—外科学] Q254[医药卫生—临床医学]