Inhibitory effects of praeruptorin C on cattle aortic smooth muscle cell proliferation  

前胡丙素对牛主动脉平滑肌细胞增殖的抑制作用

作　　者：魏恩会[1] 饶曼人[1] 陈秀英[2] 范乐明[2] 陈琪[2] 

机构地区：[1]南京医科大学心血管药理研究室,南京中国210029 [2]南京医科大学动脉粥样硬化研究中心,南京中国210029

出　　处：《Acta Pharmacologica Sinica》2002年第2期129-132,共4页中国药理学报（英文版）

基　　金：Project supported by the National Natural Science Foundation of China, № 39870363.

摘　　要：To study the effects of praeruptorin C (pra-C) on proliferation of cattle aortic smooth muscle cells ( SMC). METHODS: The DNA synthesis of SMC was measured using the incorporation of [3H] thymidine ([3H ] TdR). Cell cycle phase was evaluated by flow cytometry and cytotoxicity was evaluated by measuring lactic dehy-drogenase (LDH) activity. RESULTS: Whether or not treated with angiotensin Ⅱ (AngⅡ ), SMC proliferation was suppressed by pra-C in a concentration-dependent manner at range from 0. 001 μmol/L to 10μmol/L. The inhibitory effects appeared to be related to Gl S block in cell cycle traverse while the LDH activities did not change dramatically. CONCLUSION: Pra-C can completely inhibit SMC proliferation induced by Ang Ⅱand partly inhibit the growth of SMC induced by bovine serum, which is important in prevention and treatment of vascular hyperplastic disease.目的：探讨前胡丙素（pra-C)对牛主动脉平滑肌细胞（SMC）增殖的影响。方法：细胞DNA的合成量由^3H－胸腺嘧啶核苷[(^3H)TdR)掺入试验测定，应用流式细胞术测定细胞周期，用乳酸脱氢酶活性测定观察药物的毒性。结果：无论有无血管紧张素Ⅱ（AngⅡ），在0.001μmol/L到10μmol/L浓度范围内，pra-C均可呈浓度依赖性地抑制SMC的增殖，抑制作用主要与阻止细胞进入有丝分裂期有关，而与细胞毒性无关。结论：pra-C能够完全阻断AngⅡ诱导SMC的增殖效应，部分阻止小牛血清诱导的细胞分裂，这对于血管增生性疾病的防治有重要意义。

关 键 词：praeruptorin C CATTLE AORTA smooth muscle cell division 

分 类 号：R96[医药卫生—药理学]