意大利蜜蜂刚出房与性成熟雄蜂的蛋白质组差异分析  

Proteomic comparison between the newly emerged drones and the sexually mature drones of Apis mellifera ligustica( Hymenoptera: Apidae)

作　　者：吴小波[1] 李淑云[1] 颜伟玉[1] 曾志将[1] 

机构地区：[1]江西农业大学蜜蜂研究所,南昌330045

出　　处：《昆虫学报》2015年第3期256-263,共8页Acta Entomologica Sinica

基　　金：国家自然科学基金项目(31060327);江西省科技支撑计划项目(20141BBF60033);江西省教育厅科技计划项目(GJJ13280);国家蜂产业技术体系资助项目(CARS-45-kxj12)

摘　　要：【目的】对意大利蜜蜂Apis mellifera ligustica刚出房雄蜂与性成熟雄蜂的蛋白质组进行比较,探讨雄蜂在性成熟过程中蛋白质表达变化,为进一步研究雄蜂发育生物学获得差异表达蛋白质方面的依据。【方法】采用双向电泳法建立意大利蜜蜂雄蜂发育过程中刚出房时与性成熟期的蛋白质表达谱,通过质谱分析与数据库检索,鉴定部分差异蛋白。【结果】在意大利蜜蜂刚出房雄蜂和性成熟雄蜂中分别检测到2 490和2 317个蛋白点,其中差异表达蛋白点有157个。在刚出房雄蜂中高度表达的蛋白点有102个;在性成熟雄蜂中高度表达的蛋白点有55个。对部分差异蛋白进行质谱分析,共鉴定了18个蛋白点,其中在刚出房雄蜂中上调表达的蛋白有肌钙蛋白、SEC13蛋白、DJ蛋白等,在性成熟雄蜂中上调表达的蛋白有副肌球蛋白、精氨酸激酶、肌动蛋白解聚因子等。【结论】意大利蜜蜂雄蜂在性成熟发育过程中,其体内大量蛋白表达发生了变化,其差异表达的蛋白质可能与骨骼、飞行肌以及精子发育等机能有关。【Aim】This study aims to investigate the differentially expressed proteins between the newly emerged drones and the sexually mature drones of Apis mellifera ligustica through comparing the proteome profilings of drones at these two different stages,so as to provide the basis for further studies on the drone developmental biology. 【Methods】Two-dimensional gel electrophoresis（ 2-DE） was used to establish the proteomic map of the two kinds of drones,and part of differentially expressed proteins were identified by mass spectrometry analysis and database searching. 【Results】The results showed that 2 490 and 2 317 proteins were detected in the newly emerged drones and the sexually mature drones of A. mellifera ligustica,respectively,of which 157 differentially expressed protein spots were found. Furthermore,102 and 55 proteins were significantly up-expressed in the newly emerged drones and the sexually mature drones, respectively. Eighteen of the differentially expressed proteins were identified by mass spectrometry analysis. Among them,the Troponin,SEC13 protein homolog and DJ protein were upexpressed in the newly emerged drones while the paramyosin, arginine kinase and cofilin / actindepolymerizing factor homolog were up-expressed in the sexually mature drones. 【Conclusion】A large number of proteins in drones of A. mellifera ligustica show expression changes during maturing,which might be related to the development of cuticle,flight muscles and sperms.

关 键 词：意大利蜜蜂 雄蜂 发育 蛋白质组 差异表达蛋白 

分 类 号：Q966[生物学—昆虫学]