吡咯烷二硫代氨基甲酸盐对急性辐射损伤小鼠的防护作用  

The protective effect of pyrrolidine dithiocarbamate on acute radiation injury in mice

作　　者：靳瑾[1] 白佳利[2] 龙伟[1] 沈秀[1] 徐文清[1] 周则卫[1] 

机构地区：[1]北京协和医学院中国医学科学院放射医学研究所,天津300192 [2]山西省长治市妇幼保健院

出　　处：《中华放射医学与防护杂志》2015年第4期257-260,共4页Chinese Journal of Radiological Medicine and Protection

摘　　要：目的 研究吡咯烷二硫代氨基甲酸盐（pyrrolidine dithiocarbamate,PDTC）对急性辐射损伤小鼠的防护作用.方法 6～8周龄雄性ICR小鼠,按体重随机分为5组,每组10只：空白单照组（IR）、阳性对照组（氨磷汀WR-2721 250 mg/kg）和PDTC30、60、90 mg/kg剂量组,照射前30 min给予相应药品.^137Cs γ射线7.5 Gy一次性全身照射,观察小鼠30 d存活率;^137Cs γ射线5.0 Gy一次性全身照射,照射后第8天检测外周血、造血系统、脏器系数指标.结果 PDTC可以提高小鼠30 d存活率,60 mg/kg剂量组效果最为明显,存活率提高到60％ （6/10）,空白单照组为10％ （1/10）,阳性对照组为70％ （7/10）.与空白单照组相比,PDTC 60 mg/kg剂量组的脾脏指数、白细胞、血红蛋白含量、血小板数、股骨有核数和脾结节数,差异有统计学意义（t=2.354、4.793、2.342、6.542、2.649、3.982,P＜0.05）.结论 PDTC具有抗辐射损伤作用,尤以60 mg/kg作用最为明显.Objective To study the protective effect of pyrrolidine dithiocarbamate （PDTC） on acute irradiated mice.Methods The 6-8 weeks old male ICR mice were randomly divided into five groups：irradiation alone group （IR）,positive control group （amifostine WR-2721 250 mg/kg） and PDTC of 30,60 and 90 mg/kg dose groups.Each group had 10 mice and the drug was given at 0.5 h before whole body irradiation.At 30 d post-irradiation of 7.5 Gy ^137 Cs γrays,the mice survival were observed.At 8 d post-irradiation of 5.0 Gy ^137 Cs γ-rays,the peripheral blood,hematopoietic system and organ indexes were observed to evaluate the radiation protective effect of PDTC.Results PDTC increased the 30-day survival rates and 60 mg/kg dose had the most obvious effect by increase the survival to 60% （6/10）.The survivals of irradiation alone group and the amifostine positive control group was 10% （1/10） and 70% （7/10）,respectively.Compared with the irradiation alone group,60 mg/kg PDTC group had the significant difference in spleen index,WBC,HGB,PLT,bone marrow nucleated cells and colony forming unit of spleen （t =2.354,4.793,2.342,6.542,2.649,3.982,P 〈 0.05）.Conclusions PDTC is effective in radiation protection with an optimum dose of 60 mg/kg.

关 键 词：吡咯烷二硫代氨基甲酸盐 Γ射线 ICR小鼠 辐射损伤 

分 类 号：R965[医药卫生—药理学]