ApoE^-／-小鼠腹主动脉粥样硬化MRI及Tenascin—C、CD68表达的实验研究  

MR imaging of the atherosclerosis and the expression of tenascin-C and CD68 in ApoE/ mouse model

作　　者：孟欢 

机构地区：[1]南京医科大学第一附属医院放射科,江苏南京210029

出　　处：《实用放射学杂志》2015年第4期663-666,共4页Journal of Practical Radiology

基　　金：江苏省自然科学基金(BK20131446);国家自然科学基金(81471723).

摘　　要：目的：探讨小鼠腹主动脉粥样硬化(AS)模型7.0T MRI 成像的可行性,检测 AS 斑块中 Tenascin-C(TN-C)的表达,并探讨其与 CD68的相关性,为进一步研究其在 AS 发生发展中靶向成像的可行性奠定试验研究基础。方法高脂饲养法建立实验组ApoE^-/-小鼠和对照组野生型 C57小鼠腹主动脉 AS 模型(n=10),14周后行 MRI 检查,后处死动物取腹主动脉,行 HE 染色观察血管病理学变化,并分别使用油红 O、anti-CD68及 TN-C 抗体染色观察斑块脂质分布情况及 CD68、TN-C 的表达。结果建模14周后,MRI 见实验组腹主动脉血管壁增厚,在 T1 WI、PDWI 呈高信号,T2 WI 呈稍低信号；病理学检查见血管内膜明显增厚,管腔不规则狭窄；TN-C 及 CD68在斑块内均高表达,且二者表达区域相一致；对照组腹主动脉 MRI 未见管强狭窄,病理检查均未见内膜增厚。结论高脂饲养法可快速建立 ApoE^-/-小鼠 AS 模型,7.0T MRI 可成功检测之；TN-C 在 AS 斑块中高表达且与 CD68关系密切,二者可能通过相互协同,在 AS 发生发展中共同起作用。检测 TN-C 的表达,将有助于 AS 病变的进一步研究。Objective To explore the feasibility of 7.0T MR scanner in mouse aorta atherosclerosis models.Visualising the TN-C in atherosclerotic plaque by immunohistochemistry and its correlation with CD68 to provide experimental basis for the feasibility of TN-C in targeted MRI.Methods ApoE^-/- mice and wild type C57 mice were fed on high fat diet to establish aorta atherosclerosis model (n=10),the aorta were observed by MRI after 14 weeks.The aorta specimens were taken to stain with HE to observe the pathological changes.The plaque was stained with oil red O,anti-TNC and TN-C antibody respectively to observe the fat,CD68 and TN-C in plaque.Results 7.0 MRI showed the aortic wall of the experimental group was thicker,high signal on T1 WI and PDWI,and low signal on T2 WI after 14 weeks.The histopathlogic examination showed the intima was obviously thicker,and the lumen was ir-regulary narrow.Both of CD68 and TN-C were highly expressed in plaque,and the distribution of TN-C correlated with CD68.In the control group,no case showed hyper-signal in the vessel wall of aorta or narrow lumen by MRI,and the histopathlogy showed no for-mation of atherosclerotic plaque in the aorta.Conclusion Aorta atherosclerotic plaque can be established through high fat diet on ApoE^-/- mouse,and 7.0 MR can successfully detect it.TN-C is high expressed in AS plaque and the expression is correlated with CD68,which may suggest that they may collaborate in the development of AS.Detecting TN-C could be useful for the further study of atherosclerotic plaque.

关 键 词：动脉粥样硬化 磁共振成像 TENASCIN-C CD68 

分 类 号：R541[医药卫生—心血管疾病] R445.2[医药卫生—内科学]