氮掺杂碳量子点的合成、表征及其在细胞成像中的应用  

Synthesis,Characterization and Cell Imaging Application of Nitrogen-doped Carbon Quantum Dots

作　　者：李晓峰[1] 周明[1,2] 龚爱华[3] 张严[1] 杨青峰[1] 

机构地区：[1]江苏大学光子制造与技术中心,江苏镇江212013 [2]清华大学摩擦学国家重点实验室,北京100084 [3]江苏大学基础医学与医学技术学院,江苏镇江212013

出　　处：《材料科学与工程学报》2015年第1期41-45,121,共6页Journal of Materials Science and Engineering

基　　金：国家973计划资助项目(2011CB013004);国家自然科学基金资助项目(50975128);清华大学摩擦学重点实验室重点资助项目(SKLT10A02)

摘　　要：以葡萄糖和甘氨酸为混合碳源,在较低温度下经水热法一步合成了氮掺杂的荧光碳量子点(N-CQDs),然后对氮掺杂碳量子点的形貌、结构、组成、光学性质和细胞毒性进行了表征,最后将其应用于细胞成像。实验结果表明,对碳量子点进行氮掺杂能有效提高其荧光量子产率,其荧光增强是由于表面形成了大量强供电子基团,当葡萄糖和甘氨酸的质量比为2∶1时能获得最高为6.57%荧光量子产率。氮掺杂碳量子点还具有水溶性好、粒度均匀、优异的光致发光性质、低的细胞毒性、多波长成像等诸多优点,有望作为荧光探针应用于细胞成像等领域。Nitrogen-doped carbon quantum dots（N-CQDs）have attracted great interest due to their extraordinary properties,especially their enhanced emission efficiency and thus a facile synthesis of N-CQDs with high emission efficiency is critical for practical applications.Herein,N-CQDs were prepared via one-step hydrothermal treatment using hybrid carbon source（glucose and glycine）at a mild temperature.Morphology,structure,components,and cytotoxicity of the as-prepared N-CQDs were characterized by different instruments.It was found that Nitrogen-doping is an effective method to improve the quantum yield（QY）.The enhanced emission efficiency of N-CQDs is due to the formation of strong electron donating groups on their surface.The optimum mass ratio of glucose/glycine is 2∶1,and the QY is about 6.57%.The N-CQDs exhibit good water-solubility,well mono-dispersed,good photostability,good salt tolerance and relatively high QY.Furthermore,the N-CQDs also exhibit low toxicity and multicolor imaging properties.All these excellent properties make N-CQDs being a useful candidate of probes for cell imaging.【Key Words】： Carbon quantum

关 键 词：碳量子点 氮掺杂 细胞成像 

分 类 号：O613.71[理学—无机化学] O433.2[理学—化学]