亚砷酸钠对Hacat细胞GSK-3β及CyclinD1表达的影响  

Influence of arsenic exposure to GSK-3β and CyclinD1 in HaCat cells

作　　者：胡楠楠[1] 李昕[1] 

机构地区：[1]中国医科大学公共卫生学院劳动卫生教研室,"砷生物学作用评价及砷中毒防治"辽宁省重点实验室,辽宁沈阳110001

出　　处：《中国地方病防治》2015年第2期81-83,共3页Chinese Journal of Control of Endemic Diseases

基　　金：辽宁省教育厅课题(编号L2010705)

摘　　要：目的探讨亚砷酸钠对人上皮角质形成细胞系(Hacat细胞)PI3K/AKT信号通路中细胞周期相关分子GSK-3β、CyclinD1的影响。方法将人Hacat细胞暴露于浓度分别为0、10、25、50、100μmol/L的亚砷酸钠6 h,采用Western Blot法检测细胞总蛋白的GSK-3β,磷酸化GSK-3β(p-GSK-3β),CyclinD1和磷酸化CyclinD1(p-CyclinD1)的蛋白表达水平。结果与对照组比较,10、25、50、100μmol/L染砷组总蛋白中p-GSK-3β和CyclinD1蛋白表达水平均显著增高(P<0.05),而p-CyclinD1蛋白表达水平显著降低(P<0.05)。结论亚砷酸钠可影响Hacat细胞中的GSK-3β分子,使其磷酸化失活,进而失去对细胞周期相关蛋白CyclinD1的磷酸化作用,从而促进CyclinD1的表达。Objective To explore the influence of arsenic exposure to human Hacat cells on PI3K/AKT cell signaling pathway and its downstream of the cell cycle related molecular GSK-3β and CyclinD1. Methods HaCat cells were exposed to arsenic （ 0, 10, 25, 50,100μmol/L） for 6 hours, using western blot assay to detect the protein expression levels of GSK-3β, p-GSK -3β, and CyclinD1 and p-CyclinD1. Results Compared with those in the control group, the protein expression levels of p- GSK-3β and CyclinD1 of the test group （ 10, 25, 50, 100μmol/L） were significantly higher than that in the control group （ P 〈0.05 ）. And levels of p-CyclinD1 were significantly lower than that in the control （ P 〈0.05 ）. Conclusion Arsenic exposure can influence the GSK-3β in the Hacat cells and inactivate its phosphorylation, then lose the cell cycle related protein phosphorylation of CyclinD1 and promote the expression of cell cycle related protein CyclinD1.

关 键 词：亚砷酸钠 HACAT GSK-3Β CYCLIND1 

分 类 号：R599.9[医药卫生—内科学]