补体C5基因多态性与维吾尔族饲鸽者肺易患性的关系研究  

Relationship Between C5 Gene Polymorphism and Susceptibility to Pigeon Breeder's Lung in Uygur People

作　　者：彭玲[1] 邬超[2] 杨晓红[2] 

机构地区：[1]石河子大学医学院临床系,新疆石河子市832002 [2]新疆维吾尔自治区人民医院呼吸与危重症医学科

出　　处：《中国全科医学》2015年第14期1667-1670,共4页Chinese General Practice

基　　金：国家自然科学基金资助项目(81260003)

摘　　要：目的探讨补体C5基因多态性与维吾尔族饲鸽者肺(PBL)易患性的关系。方法选取2012年1月—2013年12月在喀什、和田地区饲养鸽子年限≥1年并在新疆维吾尔自治区人民医院呼吸科病房和门诊确诊的维吾尔族PBL患者为病例组(n=32),选择饲养鸽子年限≥1年的维吾尔族未患PBL者为阴性对照组(n=30),选择同期未饲养鸽子且本院体检健康的维吾尔族人群为正常对照组(n=30)。抽取受试者外周血抗凝,提取白细胞DNA,采用Mass ARRAY Assay Design实时PCR法进行C5基因14个单核苷酸多态性(SNP)位点检测,对存在等位基因的SNP位点进行组间比较。结果 C5基因rs17611、rs17612、rs17216529存在基因多态性,其中rs17611、rs17216529位点存在GG、AA、GA 3种基因型,rs17612位点存在CC、AA、CA 3种基因型。3组C5基因rs17611、rs17216529、rs17612基因型及等位基因频率比较,差异均无统计学意义(P>0.05)。结论补体C5基因多态性可能不影响维吾尔族PBL的发病风险。Objective To evaluate the association between C5 gene polymorphism and susceptibility to pigeon breeder＇s lung（PBL）in Uygur people. Methods We enrolled 32 Uygur patients who had bred pigeons for more than one year,came from KashiRegon and Hetian,and were diagnosed with PBL in the outpatient clinic and the Department of Pneumology,the People＇s Hospital of Xinjiang from January 2012 to December 2013 as the case group. 30 Uygur who had bred pigeons for more than one year and weren＆#39;t diagnosed with PBL,were enrolled as negative control group. Another 30 healthy Uygur people who hadn＆#39;t bred pigeons and received physical examination in the hospital in the same period were enrolled as the normal control group. Peripheral blood was sampled from the subjects,and leucocyte DNA was extracted from the samples. We conducted tests on 14 single nucleotide polymorphism（ SNP）loci in C5 genes by real - time polymerase chain reaction（ PCR）method of MassARRAY Assay Design. The three groups were compared in the SNP loci with allelic genes. Results Gene polymorphism was found in the loci of rs17611,rs17612 and rs17216529. Genotypes of GG,AA and GA were found in rs17611 and rs17216529;genotypes of CC, AA and CA were found in rs17612. No significant differences were found in rs17611, rs17216529 and rs17612 in genotype and allele frequency among three groups （ P 〉 0. 05 ）. Conclusion C5 gene polymorphisms may not have on influence on the susceptibility to PBL in Uygur people.

关 键 词：养鸟者肺 肺泡炎 外源性变应性 补体C5 多态性 单核苷酸 疾病遗传易感性 维吾尔族 

分 类 号：R563.19[医药卫生—呼吸系统]