TetR家族转录调控基因tfpA对链霉菌合成A21978C的影响  

Influence of Tet R family transcriptional regulatory gene tfp A on A21978C production in Streptomyces sp.

作　　者：逄丽[1,2] 魏维[2,3] 靳旭[2,3] 戈梅[2,3] 陈代杰[1] 

机构地区：[1]华东理工大学,上海200237 [2]上海来益生物药物研发中心有限责任公司,上海201203 [3]上海交通大学药学院,上海200240

出　　处：《中国抗生素杂志》2015年第5期334-337,368,共5页Chinese Journal of Antibiotics

基　　金：国家自然科学基金青年基金(No.81102354)

摘　　要：目的研究A21978C产生菌中一个Tet R家族转录调控基因tfp A对A21978C产量的影响。方法利用同源重组方法敲除该tfp A基因,HPLC检测突变株A21978C产量的变化,通过过表达与回补实验验证其调控特性。结果敲除tfp A基因的突变株A21978C产量与亲株相比增加了50%;基因回补后A21978C产量恢复到亲株的水平。过表达突变株则几乎不产A21978C。结论Tet R家族转录调控基因tfp A对A21978C的合成至关重要,是一个负调控基因。Objective To discover some relationships between tfpA and the biosynthesis ofA21978C which belongs to the TetR-family, a transcriptional regulator. Methods tfpA was deleted by homologous recombination, and the production of A21978C in the mutants was detected via HPLC. Then complementation and over-expression were used to confirm the function of this gene. Results Compared to the original strain, production of A21978C in the AtfpA mutant increased obviously. While the O-tfpA mutant lost its ability to produce A21978C, and C-tfpA mutant was at a similar level to that of the original strain. Conclusion tfpA exerts a strong influence on A21978C production and is a negative regulatory gene.

关 键 词：A21978C TetR家族 转录调控子 基因敲除 

分 类 号：Q93[生物学—微生物学]