24孔板培养活跃链霉菌合成那西肽条件的优化  

Optimization of nosiheptide fermentation condition in 24-well microplates by Streptomyces actuosus

作　　者：周扬[1] 薛正莲[1] 秦艳飞 赵世光[1] 刘艳[1] 

机构地区：[1]安徽工程大学生物与化学工程学院,芜湖241000 [2]安徽省皖北药业股份有限公司,宿州234000

出　　处：《中国抗生素杂志》2015年第5期344-349,共6页Chinese Journal of Antibiotics

基　　金：安徽省高校自然科学基金(No.KJ2013A048)

摘　　要：目的优化24孔板培养活跃链霉菌XW产那西肽的发酵条件。方法采用单因素实验和响应面法对24孔板的发酵条件进行优化。结果得到活跃链霉菌XW 24孔板发酵产那西肽的最优发酵条件为:种子培养基装液量2.55 m L,发酵培养基装液量0.93m L,发酵时间121h。结论通过对发酵条件的优化,可以明显提高24孔板发酵产那西肽的效价达到882.25μg/m L,实验值与模型预测值较接近,吻合度较高,较初始效价675.63μg/m L提高了30.58%。研究结果表明24孔板发酵与摇瓶发酵之间有良好的相关性,相关系数R为0.883。Objective To optimize the fermentation condition of Streptomyces actuosu XW producing nosiheptide in 24-well microplates. Methods The fermentation condition in the 24-well microplates was determined by using the single-factor experiments and the response surface method. Results The optimized fermentation condition of Streptomyces actuosu XW in 24-well microplates was obtained as follows： the liquid volume of seed medium 2.55mL, the liquid volume of fermentation medium 0.93mL and fermentation time 121h respectively. Conclusion The production of nosiheptide in 24-well microplates was improved significantly under the optimal condition, reaching 882.25ktg/mL, the experimental value was comparable to model prediction, having high inosculating degree, which was increased by 30.58% compared with the original condition. The result showed that there was a highly significant correlation relationship between the A408 of 24-well microplates and the A408 of shake flasks. The correlation coefficient R between the A408 of 24-well microplates and the A408 of shake flasks was 0.883.

关 键 词：那西肽 活跃链霉菌 响应面优化 24孔板 

分 类 号：R978.1[医药卫生—药品]