重瓣榆叶梅花中蛋白质提取工艺及动态含量研究  

Optimal Extraction Technology and Dynamic Content of Protein in the Flower of Amygdalus triloba f.multiplex

作　　者：徐毅励 陈媛梅[1] 

机构地区：[1]北京林业大学理学院,北京100083

出　　处：《西北林学院学报》2015年第3期191-196,共6页Journal of Northwest Forestry University

基　　金：国家自然科学基金(30671655)

摘　　要：重瓣榆叶梅花中蛋白质提取研究表明,最佳提取工艺为采用pH7.0的磷酸氢二钠提取液、液料比为30∶1(mL∶g)、30℃、500 W、超声提取3次(每次120min)。在该提取工艺条件下,测得开花14d花中蛋白质含量最高,为13.237mg·g-1;开花21d花中蛋白质含量最低,仅为5.150mg·g-1。Crude proteins were extracted from the flowers of Amygdalus triloba f. multiplex. The optimal conditions of the extraction were obtained： Solvent： disodium hydrogen phosphate with pH= 7.0, ration of solvent to material.-30 ： l（mL ： g）. The extraction was conducted for 3 times （120 min each） at 30℃ aided by ultrasonic wave （500 W of ultrasonic generator power）. By above extraction conditions, it was found the highest content of protein was in the flowers collected at the 14th day after blossoming, about 13. 237 mg·g^-1 , lowest at the 21st day, about 5. 150 mg·g^-1 .

关 键 词：重瓣榆叶梅花 超声提取 蛋白质 液料比 动态含量 

分 类 号：S713[农业科学—林学]