离线二维液相色谱串联质谱法检测东北刺人参根中的化学成分  

Separation and Identification of the Chemical Constituents from the Roots of Oplopanax Elatus by Using Off-line Liquid Chromatography Tandem Mass

作　　者：聂明坤 罗伟[2] 王冲之[3] 袁钧苏[3] 黄卫华[2] 施树云[1,2] 欧阳冬生[2] 

机构地区：[1]中南大学化学化工学院,湖南长沙410083 [2]中南大学湘雅医院临床药理研究所,湖南长沙410008 [3]美国芝加哥大学唐草药研究中心,美国芝加哥il60637

出　　处：《广州化工》2015年第9期136-138,共3页GuangZhou Chemical Industry

基　　金：中央高校基金中南大学基本科研业务费(1681-7608040003)

摘　　要：利用离线二维液相色谱串联高分辨质谱的方法分析检测东北刺人参根的化学成分,以CN色谱柱为第一维分离制备组分,ODS(C18)色谱柱为第二维色谱分析,结果表明,构建的二维液相体系峰容量明显提高,共检测得到31个成分,并结合UV及HRMS信息鉴定出其中7个化合物,分别为:松柏苷(1),紫丁香苷(2),(+)-异落叶松脂素-3-α-O-β-D-葡萄糖苷(3),oploxynes A(4),oplopantriol A(5),(3S,8S)-falcarindiol(6)和oplopandiol(7)。Two-dimensional liquid chromatography-diode array detection-mass spectrometry （2D-LC-DAD-MS） assay was developed for separation and identification of the chemical constituents from the roots of Oplopanax elatus. Eight compounds were identified or tentatively deduced based on their retention time, UV, accurate molecular weight. The results demonstrated that the method can provide a considerable improvement in peak capacity compared with the traditional 1D-HPLC.

关 键 词：东北刺人参 二维液相色谱 高分辨质谱 

分 类 号：O657.7[理学—分析化学]