转LjCYC2基因改变花生花瓣对称性的初步研究  

Preliminary Studies on Transformation of Peanut(Arachis hypogaea L.) with Flower Symmetry Gene LjCYC2 in Lotus Japonicus

作　　者：温世杰[1] 刘海燕[1] 洪彦彬[1] 黄霞[2] 梁炫强[1] 

机构地区：[1]广东省农业科学院作物研究所广东省农作物遗传改良重点实验室,广东广州510640 [2]中山大学生命科学学院广东省热带亚热带植物资源重点实验室,广东广州510275

出　　处：《热带作物学报》2015年第5期883-887,共5页Chinese Journal of Tropical Crops

基　　金：“十二五”国家科技支撑计划课题(No.2013BAD01B0702);现代农业产业技术体系建设专项资金(No.CARS-14);粤港关键领域重点突破项目(No.200849863);广东省农业科学院作物研究所/广东省农作物遗传改良重点实验室开放基金项目;广东省科技基础条件建设项目(No.2013B060400034)

摘　　要：花生闭花传粉是花生杂交育种的障碍,人工去龙骨瓣是花生杂交成功的关键。通过转入Lj CYC2基因,在一定程度上改变了花生的花型,结果表明在变异花中,天然去龙骨瓣的花在T1代的变异率为0.01%,在T2代的变异率提高到1.1%。It was an obstacle of peanuts cross breeding, which was clistogamy. And manual removal of keel was the key to peanut hybridization successful. In this study, flower patterns of peanuts were changed by LjCYC2 gene transformed. The results showed that the mutation rate of natural no keel petals was 0.01% in the T1 generation and 1.1% in the T2 generation. These researches lay a good foundation for acquiring stable peanut species which are natural no keel and used in heterosis of peanut in the future.

关 键 词：花生 LJ CYC2基因 遗传转化 

分 类 号：S565.2[农业科学—作物学]