山苍子雌花和雄花精油成分分析及抗氧化研究  

Composition Analysis and Antioxidant Research of Essential Oils from Litsea cubeba Female and Male Flower

作　　者：李欣欣[1] 余雪芳[1] 黄添毅[1] 曹莉莉[1] 吴少华[1] 李永裕[1] 

机构地区：[1]福建农林大学园艺学院,福建福州350002

出　　处：《热带作物学报》2015年第5期942-948,共7页Chinese Journal of Tropical Crops

基　　金：高等学校博士学科点专项科研基金(No.20113515120016);福建省自然科学基金项目(No.2011J05050);福建省教育厅科技计划项目(No.JK2012016);福建农林大学园艺学院青年学术骨干培养基金(No.FAFU2012YYPY03)

摘　　要：采用水蒸气蒸馏法分别从山苍子雌花和雄花中提取精油,并通过GC-MS气质联用仪对其成分分析,从山苍子雌花精油中鉴定出47种化合物,占总峰面积的98.44%,山苍子雄花精油中鉴定出43种化合物,占总峰面积的98.04%。采用体外抗氧化活性测定法,表明山苍子雌花和雄花精油具有一定的抗氧化活性。还原力测定IC50值,雄花为2.330 mg/m L,雌花为1.473 mg/m L;清除DPPH自由基IC50值,雄花为41.62 mg/m L,雌花为9.663 mg/m L;清除羟自由基IC50值,雄花为56.95 mg/m L,雌花为77.98 mg/m L。By steam distillation, extract essential oils from Litsea cubeba female and male flowers respectively, and analyzing its composition by GC-MS. Female flower identified 47 compounds in essential oil, accounted for 98.44% of total peak area, male flower 43 compounds identified in the essential oils, accounting for 98.04% of the total peak area. Through determination of antioxidant activity in vitro, showed that essential oil from Litsea cubeba female and male flowers has a certain antioxidant activity. Reducing power ICso value concentration of male flowers was 2.330 mg/mL, female flowers is 1.473 mg/mL; Remove DPPH free radicals IC50 value concentration of male flowers was 41.62 mg/mL, female flowers is 9.663 mg/mL; IC50 value concentration of hydroxyl free radicals, male flowers is 56.95 mg/mL, female flowers is 77.98 mg/mL.

关 键 词：山苍子 精油 气相色谱-质谱(GC-MS) 抗氧化 

分 类 号：S565.9[农业科学—作物学]