多基因序列鉴定油茶炭疽病原Colletotrichum boninense新种  

Identification of a New Anthracnose of Camellia oleifera Based on Multiple-gene Phylogeny

作　　者：汤铱泠[1] 周国英[1] 李河[1] 钟文斌[2] 龚洪恩[2] 王丽云[2] 

机构地区：[1]中南林业科技大学经济林培育与保护教育部重点实验室,湖南长沙410004 [2]中国林业科学研究院亚热带林业实验中心,江西分宜336600

出　　处：《热带作物学报》2015年第5期972-977,共6页Chinese Journal of Tropical Crops

基　　金：林业公益性行业科研专项(No.201304403);国家自然科学基金(No.31100479)

摘　　要：油茶炭疽病是中国油茶最重要的病害之一,为进一步对油茶炭疽病原进行研究,通过柯赫氏法验证,从海南澄迈和江西分宜的油茶叶上分离到有别于其它炭疽病菌的致病菌3株。菌株在PDA培养基上,菌落圆形,呈奶油色至橙黄色,菌丝生长速度为10.8-11.3 mm/d;附着胞为椭圆形,呈深褐色至棕黑色,边缘整齐,大小为（5.3±0.9）μm×（16.1±1.1）μm;分生孢子初为橘黄色,后形成黑色孢子团,大小为（14.5±2.5）μm×（5.6±1.6）μm。多基因系统发育树显示3株病原菌与博宁炭疽菌（Colletotrichum boninense）的模式株MAFF 305972聚为一个进化枝,且达到100%的置信度。结合其形态和多基因分析,结果表明：3个菌株为博宁炭疽菌（C.boninense）,是能侵染油茶树的炭疽菌种。Camellia oleifera anthracnose is one of the most important plant diseases in China. In order to further sdudy the anthracnose, samples of diseased leaves were collected form Fenyi, Jiangxi and Chengmai, Hainan.Three strians different from other strains which could infect the leaves were obotained. Their colonies on PDA media were round with color from creamy to aurantiacus, and the mycelium growing speed was 10.8-11.3 mm/d;The appressorium from the mycelia was round, puce to sepia, neat edges,（5.3±0.9）μm×（16.1±1.1）μm sized; The conidial spores were pink first then appeared to black spore balls,（14.5 ±2.5）μm×（5.6±1.6）μm sized. The multigene phylogenetic tree system showed that the 3 pathogenic strains and the typestrain of Colletotrichum boninense clustered into one clade, the confidence level was 100%. Utilizing morphology and multi-gene analysis to indentify the pathogens, the results indicated that they belonged to C. boninense and they were a new recorded anthrax pathogen which could infect C. oleifera.

关 键 词：油茶 炭疽病 多基因 博宁炭疽菌 

分 类 号：Q939.95[生物学—微生物学] S794.4[农业科学—林木遗传育种]