别嘌呤醇对肠道缺血再灌注损伤的保护作用  

Protective effect of allopurinol against intestinal ischemia-reperfusion injury

作　　者：代小娟[1] 杨涛[1] 许美霞[1] 曹好好[1] 张晓霞[1] 毕传华 许涛[1] 

机构地区：[1]武汉市普爱医院重症医学科,湖北省武汉市430015

出　　处：《世界华人消化杂志》2015年第11期1796-1800,共5页World Chinese Journal of Digestology

摘　　要：目的:探讨别嘌呤醇在肠道缺血再灌注中的保护作用.方法:根据随机数字表法将30只♂SD大鼠分为假手术组、别嘌醇组和生理盐水组,每组10只.参照Koi Ke等方法复制肠模型,采用比色法测定二胺氧化酶(diamine oxidase,DAO)水平、丙二醛(malondialdehyde,MDA)含量及黄嘌呤氧化酶(xanthine oxidase,XO)活性.应用SPSS16.0统计软件进行分析,P<0.05为差异有统计学意义.结果:3组大鼠回肠黏膜Chiu氏评分比较,差异有统计学意义(P<0.05);而别嘌呤醇组评分则与生理盐水组比较明显降低(P<0.05),但仍高于假手术组(P<0.05).3组大鼠血清DAO水平不同,差异有统计学意义(P<0.05);别嘌呤醇组大鼠血清DAO水平显著高于假手术组(P<0.05);与生理盐水组比较,别嘌呤醇组血清DAO水平明显降低(P<0.05).3组大鼠回肠组织中MDA含量比较,差异有统计学意义(P<0.05);别嘌呤醇组大鼠回肠组织中MDA含量明显高于假手术组(P<0.05);显著低于生理盐水组(P<0.05).3组大鼠回肠组织中XO活性测定比较,差异有统计学意义(P<0.05);别嘌呤醇组回肠组织中XO活性测定与假手术组比较均明显升高(P<0.05);显著低于生理盐水组(P<0.05).结论:别嘌呤醇通过抗氧化、清除自由基的作用,对肠道缺血再灌注损伤有保护作用.AIM： To investigate the protective effect of allopurinol against intestinal ischemiareperfusion injury. METHODS： Thirty male SD rats were randomly divided into a sham operation group, an allopurinol group and a saline group, with 10 rats in each group. A rat model of intestinal ischemia was established. Serum content of diamine oxidase（DAO）, tissue level of malondialdehyde（MDA） and activity of xanthine oxidase（XO） were detected by colorimetry. RESULTS： Ileal mucosa Chiu’s score differed significantly among the three groups（P 〈 0.05）, and the score in the allopurinol group was significantly lower that in the saline group（P 〈 0.05）, but significantly higher than that in the sham operation group（P 〈 0.05）. Serum DAO levels differed significantly among the three groups（P 〈 0.05）, and serum DAO levels in the allopurinol group was significantly higher than that in the sham operation group（P 〈 0.05）, but significantly lower than that in the saline group（P 〈 0.05）. MDA contents in the ileum differed significantly among the three groups（P 〈 0.05）, and ileal tissue MDA in the allopurinol group was significantly higher than that in the sham operation group（P 〈 0.05）, but significantly lower than that in the saline group（P 〈 0.05）. XO activity differed significantly among the three groups（P 〈 0.05）, and XO activity in the allopurinol group was significantly higher than that in the sham operation group（P 〈 0.05）, but significantly lower than that in the saline group（P 〈 0.05）. CONCLUSION： Allopurinol has a protective effect against intestinal ischemia-reperfusion injury possibly through its antioxidant and free radical scavenging activities.

关 键 词：肠道缺血再灌注损伤 别嘌呤醇 二胺氧化酶 

分 类 号：R965[医药卫生—药理学]