灵芝酸A对癫痫样放电海马神经元的影响  

The Effects of Ganoderma Acid A on Hippocampal Neurons of Epileptic Discharges

作　　者：吴荣亮[1,2] 刘君星[1] 

机构地区：[1]佳木斯大学,黑龙江佳木斯154000 [2]鸡西矿业集团总医院,黑龙江鸡西158100

出　　处：《牡丹江医学院学报》2015年第2期8-11,共4页Journal of Mudanjiang Medical University

摘　　要：目的旨在验证灵芝酸A对海马神经元异常放电的影响。方法分离24h新生Wistar大鼠原代海马神经元。正常对照组:将培养9d的海马神经元全液换成正常细胞外液处理3h,然后恢复正常培养24h;无Mg2+诱导组、灵芝酸A组、紫杉醇组、γ-氨基丁酸组、丙戊酸钠组分别将培养9d的海马神经元全液换成无镁细胞外液或添加相应的50μg/m L的灵芝酸A、50μmol/L的紫杉醇、100μmol/L的γ-氨基丁酸、100mg/L的丙戊酸钠混悬液处理3h,然后恢复正常培养,MTT法检测细胞存活率;分光光度计检测超氧化物歧化酶活性;流式细胞仪检测细胞线粒体膜电位的变化。结果 50μg/m L灵芝酸A可提高海马神经元细胞存活率;能升高癫痫样海马神经元细胞SOD活性、升高线粒体膜电位。结论灵芝酸A可通过抑制细胞氧化损伤、抑制凋亡而保护异常放电的海马神经元。Objective To isolate rat primary hippocampal neurons , detect the impact of Ganoderma acid A on the epileptic cell mitochondrial membrane potential and superoxide dismutase in ouder to investigate the antiepileptic mechanism of Ganoderma acid A , provide clinical antiepileptic drugs theory basis.Methods 24h newborn Wistar rat primary hippocampal neurons rere isolated , and then the experimental groups were divided into normal control group： the 9th days cultured hippocampal neurons in complete medium were treated with normal extracellular fluid for 3 hours, and recovered complete medium for 24h;no Mg2＋induction group , Ganoderma acid group A , paclitaxel ,γ-aminobutyric acid group , sodium valproate group were treated with Mg 2＋-free extracellular fluid or ad-ditional added 50μg /mL Ganoderma acid A, 50μmol /L paclitaxel, 100μmol /L γ-aminobutyric acid, 100mg /L sodium val-proate suspensions for 3h, and recovered complete medium for 24h;We detect the changes of the mitochondrial membrane potential by flow cytometry, superoxide dismutase activity by spectrophotometer , to explore the pathogenesis of epilepsy and the antiepileptic mecha-nism of Ganoderma acid A.Results We successfully isolated rat primary hippocampal neurons;Ganoderma acid A at 50μg /mL con-centration were nontoxic to hippocampal neurons;Ganoderma acid A increased the activity of SOD in hippocampal neurons , Ganoderma acid A elevated the epileptic mitochondrial membrane potential.Conclusion Ganoderma acid A has anti -epileptic effect by antioxi-dant and inhibiting apoptosis.

关 键 词：灵芝酸A 海马神经元 癫痫 

分 类 号：R338[医药卫生—人体生理学]