羊栖菜多糖对高脂培养的胰岛β细胞凋亡的影响  

Effect of Sargassum Fusiforme Polysaccharide on Apoptosis of Pancreatic Beta Cells in Saturated Fatty Acids

作　　者：张杰[1] 杨骄霞[2] 詹必勋 石杰[1] 杨旭东[1] 申梅淑[1] 宋高臣[1] 王桂云[1] 田丽梅[1] 王崴 

机构地区：[1]牡丹江医学院 [2]牡丹江医学院附属红旗医院 [3]牡丹江血栓病医院,黑龙江牡丹江157011 [4]哈尔滨市中医医院,黑龙江哈尔滨150040

出　　处：《牡丹江医学院学报》2015年第2期12-14,共3页Journal of Mudanjiang Medical University

摘　　要：目的研究羊栖菜多糖(Sargassum fusiforme polysaccharide,SFPS)对在高脂培养的胰岛β细胞凋亡的影响及其分子机制。方法采用MTT法检测SFPS对胰岛β细胞增殖的影响,Hoechst 33258检测胰岛β细胞凋亡情况,反转录RCR法检测bax、caspase-3基因表达量的变化。结果与模型组比较,5%SFPS组和10%SFPS组胰岛βTc3细胞的生长抑制明显减轻(P<0.01),胰岛βTc3细胞的凋亡率明显降低(P<0.01),bax和caspase-3基因表达亦降低(P<0.01)。结论 SFPS能抑制高脂诱导的胰岛β细胞凋亡,其机制可能与调控bax和caspase-3基因表达有关。Objective To study effect and molecular mechanism of SFPS on Apoptosis of pancreatic beta cells in saturated fatty acids.Methods We observed the cells proliferation by Tetrazolium （ MTT） and cell apoptosis of βTc3 cells by Hoechst 33258.We detected the levels of bax and caspase -3 gene expression in all groups by reverse transcription PCR （ RT-PCR）.Results With com-parison to model group , proliferation inhibition rate of βTc3 cells,the apoptotic rate of βTc3 cells the level of bax and caspase -3 gene expression were significantly declined in 5%SFPS group and 10%SFPS group.Conclusion SFPS could inhibite apoptosis of βTc3 cells probably through regulating bax and caspase -3 gene expression.

关 键 词：羊栖菜多糖 BAX基因 CASPASE-3基因 细胞凋亡 ΒTC3细胞 

分 类 号：R34[医药卫生—基础医学]