造影红细胞对剪切流中肿瘤细胞黏附的影响  

Effects of ghost red blood cells on tumor cell adhesion in shear flow

作　　者：傅怡[1] 吴洁[1] 孙仁[1] 丁祖荣[1] 董澄[1,2] 

机构地区：[1]上海交通大学船舶海洋与建筑工程学院水动力学教育部重点实验室,上海200240 [2]Department of Bioengineering,the Pennsylvania State University

出　　处：《医用生物力学》2015年第2期99-103,共5页Journal of Medical Biomechanics

基　　金：国家自然科学基金项目(11302129);美国国立卫生研究院(NIH)资助项目(CA-125707);美国国家科学基金会(NSF)资助项目(CBET-0729091)

摘　　要：目的研究造影红细胞对剪切流环境中白细胞介导肿瘤细胞在内皮细胞上黏附的影响。方法在平行平板流动腔中加入20%比容的造影红细胞,分析不同剪切率(62.5、100、200 s-1)下内皮细胞上黏附白细胞数目、肿瘤细胞与黏附白细胞的碰撞事件以及稳定黏附肿瘤细胞数目的变化。结果造影红细胞促进白细胞在内皮细胞上黏附,增加肿瘤细胞与黏附白细胞的碰撞频率,并最终促进肿瘤细胞在内皮细胞上的黏附,且这一现象在高剪切率(200 s-1)下更为明显;但造影红细胞对肿瘤细胞的黏附效率并无显著影响。结论剪切流中造影红细胞的存在对肿瘤细胞在内皮细胞上的黏附起到促进作用,研究结果为探索癌症治疗方法提供理论基础。Objective To study the effect of ghost red blood cells（ GRBCs） on white blood cell（ WBC）-mediated adhesion of tumor cells（ TCs） on endothelial cells（ ECs） in shear flow. Methods GRBCs with hematocrit（ Hct）of 20% were added in the parallel plate flow chamber to observe changes in the number of tethered WBCs on ECs,the collision between TCs and adhesive WBCs,and the number of firmly adhered TCs at different shear rates of 62. 5,100,200 s^-1,respectively. Results GRBCs could increase the number of adhered WBCs on ECs and the collision between TCs and adhesive WBCs,and finally enhance the adhesion of TCs on ECs,especially at high shear rate（ 200 s^-1）. However,the adhesion efficiency of TCs was not significantly influenced by GRBCs. Conclusions GRBCs in shear flow can promote TC adhesion on ECs,and the research finding will provide a theoretical basis for cancer therapy.

关 键 词：造影红细胞 内皮细胞 白细胞 肿瘤细胞 剪切流 细胞黏附 

分 类 号：R318.01[医药卫生—生物医学工程]