番茄红素对血管紧张素Ⅱ诱导H9c2心肌细胞凋亡的影响  

Effect of iycopene on H9c2 cell apoptosis induced by angiotensin Ⅱ

作　　者：胡哲夫[1] 唐其柱[1] 刘源[1] 李金[1] 张文斌[1] 

机构地区：[1]武汉大学人民医院心血管内科,430060

出　　处：《中华老年医学杂志》2015年第5期553-556,共4页Chinese Journal of Geriatrics

基　　金：教育点博士基金（201301411300io）

摘　　要：目的研究番茄红素（Lyc）对血管紧张素Ⅱ（AngⅡ）诱导H9c2心肌细胞凋亡的保护作用，并探讨其可能的机制。方法采用AngⅡ（10μmol／L）刺激H9c2细胞，观察不同浓度Lyc对AngⅡ诱导细胞凋亡的保护效果。采用CCK8检测不同浓度Lyc和（或）AngⅡ对H9c2细胞活性的影响；采用实时半定量逆转录-聚合酶链反应（RT-PCR）检测细胞中Bax、Bcl-2的基因表达水平；免疫印迹（Westernblot）法检测细胞中Bax、Caspase3、Caspase9、Bcl-2的蛋白表达量；TUNEL染色观察细胞的凋亡比率。结果和对照组相比，AngⅡ组中H9c2细胞活性为（92．87±4．37）％，当Lyc与AngⅡ同时干预H9c2细胞后，细胞活性显著上升（P〈0．05）；RTPCR结果显示AngⅡ可上调H9c2胞内BaxmRNA表达，Bcl-2表达下调；当Ang1I与Lyc同时作用时，Bax表达下调而Bcl-2表达上调，且这种改变具有浓度依赖性；Bax、Caspase3、Caspase9在Ang1I的作用下蛋白表达量上调，Bcl-2下调（P〈0．05），而AngⅡ与Lyc同时作用时可逆转这一现象。TUNEL染色结果显示，AngⅡ作用于H9c2细胞时，可见大量凋亡细胞；而Lyc与Ang1I同时作用时，凋亡细胞的数量明显减少（P〈0．05）。结论Lyc能够明显改善AngⅡ诱导的H9c2心肌细胞凋亡，提示其对于改善临床上各种心血管疾病可能有着重要的作用。Objective To investigate the effect of lycopene （Lyc） on H9c2 cell apoptosis induced by angiotensin Ⅱ （AngⅡ ）. Methods Using Ang Ⅱ （10 〉mol/L） to stimulate H9c2 cells, we observed the protective effect of Lyc on H9c2 cells apoptosis. The H9c2 cells viability induced by different consideration of Lye or Ang 11 or both was detected by CCK8 assay. The expression levels of Bax and Bcl 2 in H9c2 cells were determined by real-time quantitative reverse transcription polymerase chain reaction （RT-PCR）. Western blot was conducted to detect the protein expressions of Bax, Caspase 3, Caspase 9 and Bcl-2 in H9c2 cells. The apoptotic ratio of H9c2 cells was observed by TUNEL assay. Results Compared with control group, AngⅡ could decrease the viability of H9c2 cells to （92.87±4.37）%. The result of RT-PCR showed that Ang Ⅱ decreased the expression level of Bcl 2, and Bax level was increased under the stimulation of AngⅡ （P〈0.05）, while the expression level of Bcl-2 was increased and Bax level was decreased under the co-stimulation of AngⅡ and Lyc in a concentration dependent manner, which indicated that Lyc ameliorated the apoptosis of H9c2 cells. The result of western blot showed that the protein expressions of Bax, Caspase 3 and Caspase 9 were increased, but Bcl-2 was decreased after the stimulation of Ang Ⅱ （P〈 0.05）. While these phenomenon reversed apparently under the co-stimulation of Ang Ⅱ and Lye. A large number of apoptotic cells were observed under the stimulation of Ang Ⅱ through TUNEL assay. But the number of apoptotic cells reduced significantly under the co-stimulation of Lyc and Ang Ⅱ （P 〈0.05）. Conclusions Lyc ameliorates the H9c2 cell apoptosis induced by Ang Ⅱ , which indicates that Lyc may have an important role in the treatment of various cardiovascular diseases.

关 键 词：番茄 血管紧张素Ⅱ 肌细胞 心脏 细胞凋亡 

分 类 号：R285[医药卫生—中药学]