人输卵管内膜干细胞和输卵管间充质干细胞生物学特性的比较研究  

Comparative study of biological properties between mucosal stem cells and mesenchymal stem cells obtained from human fallopian tube

作　　者：王娇娇[1] 王蔼明[2] 赵勇[2] 武晓云 

机构地区：[1]南方医科大学第三临床医学院,广州510515 [2]海军总医院妇产科,北京100048 [3]北京京蒙高科干细胞实验室,北京100085

出　　处：《解放军医学杂志》2015年第4期292-296,共5页Medical Journal of Chinese People's Liberation Army

基　　金："十二五"国家科技支撑计划课题(2012BAI32B05)~~

摘　　要：目的探讨人输卵管内膜中是否存在干细胞,并比较输卵管内膜干细胞(FMSC)与输卵管间充质干细胞(FTMSC)的生物学特性。方法体外分离、培养及扩增人FMSC和FTMSC,比较两种干细胞的细胞形态、细胞表型、克隆形成率、增殖能力和分化能力。结果体外分离、培养及扩增的两种干细胞均呈长梭形,细胞形态相似,但与FTMSC比较,FMSC形态更为均一,有更高的细胞累积群倍数,克隆形成检测也显示其克隆形成率较高。流式细胞术检测显示两种干细胞有相似的细胞表型,体外诱导分化结果显示两者都具有成骨、成脂肪及成软骨分化能力。结论输卵管内膜层存在干细胞,且FMSC较FTMSC有更强的增殖能力,推测FMSC较FTMSC有更高的临床应用价值。Objective To explore whether there are stem cells in the endosalpinx, and to compare the biological properties between the mucosal stem cells （MuSCs） and mesenchymal stem cells （MeSCs） obtained from the fallopian tube for the purpose of providing experimental basis for clinical application. Methods In this study, we isolated, cultured and amplified human MuSCs and MeSCs in vitro, and compared the cell morphology, cell phenotype, colony forming efficiency, proliferation and differentiation capacities of MuSCs and MeSCs. Results Both MeSCs and MuSCs showed a similar elongated morphology, but the arrangement of MuSCs were more uniform than that of MegCs. MuSCs showed a significantly higher proliferation capacity compared with MeSCs. Colony forming unit-fibroblast analysis further showed that more colonies formed with MuSCs than that of MeSCs, with a significant statistical difference. However, FTMSC and FMSC showed similar immunophenotypes. In vitro assays revealed that both types of cells showed the potential of adipogenic, osteogenic, and chondrogenic differentiation. Conclusions Stem cells were found in the fallopian tube mucosa, and MuSCs have stronger proliferation capacity than MeSCs, thus they possess a higher value for clinical application.

关 键 词：输卵管 间质干细胞 细胞培养技术 

分 类 号：R329.24[医药卫生—人体解剖和组织胚胎学]