重组质粒pVR-S与重组腺病毒rAdV-S诱导特异性免疫反应的研究  

Specific immune responses of recombinant plasmid pVR-S and recombinant adenovirus rAdV-S

作　　者：王颖慧[1] 陈国敏[1] 毕胜利[1] 曾毅[1] 

机构地区：[1]中国疾病预防控制中心病毒病预防控制所,北京100052

出　　处：《中华实验和临床病毒学杂志》2015年第2期118-120,共3页Chinese Journal of Experimental and Clinical Virology

摘　　要：目的 研究表达HBsAg的重组质粒pVR-S与表达HBsAg的重组腺病毒rAdV-S在不同免疫策略下的免疫效果.方法 使用BALB/c小鼠,在比较电击与肌注两种方法后,分别单独免疫pVR-S和rAdV-S,使用ELISA和Elispot方法分别检测体液免疫和细胞免疫水平;然后采用联合免疫pVR-S和rAdV-S,比较联合免疫与单独免疫对小鼠产生免疫应答的不同影响.结果 与肌内注射相比较,电击注射pVR-S能更快诱导较高的HBsAb抗体水平.当pVR-S和rAdV-S分别单独免疫时,pVR-S引起体液免疫应答的速度较慢,而rAdV-S可在第2周迅速诱导产生体液免疫应答,rAdV-S诱导的体液免疫应答和细胞免疫应答水平均高于pVR-S;联合免疫pVR-S和rAdV-S时,联合免疫产生的细胞免疫应答是单独免疫的2-3倍,同时还可诱导出相同或更高水平的体液免疫应答.结论 rAdV-S的单独免疫效果高于pVR-S,联合免疫可比单独免疫诱导产生更强的免疫应答,有助于提高机体抗病毒能力.Objective The objective of this study was to analyze the immune effects of pVR-S and rAdV-S with different immune strategies.Methods HBsAg gene was used to construct recombinant DNA plasmid named pVR-S and recombinant adenovirus named rAdV-S,respectively.Firstly,BALB/c mice were injected with pVR-S by two methods,including electroporation and intramuscular injection.Secondly,two groups of mice were immunized with rAdV-S and pVR-S,respectively.Level of HBsAb was detected by ELISA and cellular responses were detected by Elispot to evaluate the immune effects.Finally,DNA primeAdenovirus boost strategy was used to evaluate the humoral and cellular immune responses.Results Compared with intramuscular immunization,electroporation-mediated immunization could induce stronger humoral immune response.Independent pVR-S increased HBsAb level slowly,while independent rAdV-S induced HBsAb at week 2 with higher HBsAb level than pVR-S.What is more,pVR-S prime-rAdV-S boost strategy could result in two to three times higher cellular responses than independent immunizations.Conclusion Recombinant adenovirus rAdV-S could induce strong humoral and cellular immune responses.pVR-S prime-rAdV-S boost immunization was better than independent rAdV-S immunization,contributing to excellent antiviral capability.

关 键 词：肝炎表面抗系 乙型 腺病毒科 免疫接种 加强 

分 类 号：R392[医药卫生—免疫学]