粳稻光氧化特性筛选方法的改良及应用  

Modified Approach for Screening Photooxidation Characteristics in Japonica Rice Varieties and Its Application

作　　者：李霞[1] 霍垲 陈平波[1] 魏晓东[1] 钱宝云[1] 刘小龙[1] 范方军[1] 仲维功[1] 

机构地区：[1]江苏省农业科学院粮食作物研究所,江苏省优质水稻工程技术研究中心,南京210014

出　　处：《分子植物育种》2015年第4期748-754,共7页Molecular Plant Breeding

基　　金：国家自然科学基金项目(30871459;31371554);江苏省农业科技创新基金(CX[(12)1003]);江苏省自然科学基金项目(BK20130708)共同资助

摘　　要：为了精确鉴定粳稻材料的光氧化特性,本研究改进了光氧化筛选方法,即在人工光氧化处理条件下,将处理时间从5 d延长到10 d(如遇阴雨天气,则再顺延2 d),对64份粳稻材料的光氧化特性,进行筛选和鉴定。结果显示,用改进的方法,粳稻光氧化级别为1的类型,可进一步细化为4级,并通过叶色的黄化和处理前后叶绿素含量的变化,进行验证。之后,利用该改良方法鉴定供试材料的光氧化级别与预备试验的生长和产量的指标进行了聚类分析,以平均距离5为分类标准,可将筛选粳稻材料分为3种类型,其中类型1是产量最高的类型,平均光氧化级别为1.8级;类型3的光氧化级别为2.2,产量最低;类型2无论产量还是光氧化特性是位于上述两类型中间的类型,结果说明粳稻的光氧化特性与产量表现有类似的变化趋势。对于本研究中鉴定的类型1的粳稻材料,虽然其平均产量最高,但是其耐光氧化级别则低于类型2的,其光氧化特性还需改良。本研究可为准确鉴定粳稻的光氧化特性提供简易有效的方法。In order to screen accurately the photo-oxidation characteristics in japonica rice varieties, we modified the artificial photo-oxidative conditions. The treatment time extended from 5 days to 10 days （if it met cloudy or raining, the treatment days extended another 2 days, respectively）. Then we screened 64 japonica rice varieties under above-mentioned conditions. The photo-oxidation characteristics of these tested rice plants were divided into some grades by using the modified screening method, in which of grade 1 of photo-oxidative characteristics can be further divided into 4 levels. The modified method was validated by leaf yellowing and the chlorophyll content before and after the treatment. At last, the growth and yield indicators of their last preliminary test and the photo-oxidation characteristics by using this modified method were conducted by clustering analysis. Screening materials can be divided into 3 types according to 5, including type 1 with the highest yield and the average photo-oxidation grade of 1.8; Type 3 with the average photo-oxidation grade of 2.2 and the lowest yield;Photo-oxidation characteristics and the yield level in type 2 were lying between the Above-mentioned types. The screening results suggested that there be a similar trend between photo-oxidation grades and their yield in japonica rice varieties. As for type 1, their photo-oxidation tolerance should be to be further improved though their yield was the highest among the three types screened in this study. This method might provide a simple and effective approach for screening the photo-oxidation characteristics in the japonica rice plants.

关 键 词：粳稻 光氧化特性 筛选方法 聚类分析 

分 类 号：S511.22[农业科学—作物学]