小麦温敏不育系SCT-1育性基因初步定位分析  

Preliminary Molecular Mapping of Male Sterility Genes in Thermo-sensitive Male Sterility Wheat Line SCT-1

作　　者：郑建敏[1] 蒲宗君[1] 李式昭[1] 李俊[1] 杨恩年[1] 万洪深[1] 刘培[2] 

机构地区：[1]四川省农业科学院作物研究所,农业部西南地区小麦生物学与遗传育种重点实验室,成都610066 [2]四川省农业科学院农产品加工研究所,成都610066

出　　处：《分子植物育种》2015年第4期775-780,共6页Molecular Plant Breeding

基　　金：省财政基因工程专项资金;四川省杰出青年基金(2012JQ0066);863强优势小麦杂交种的创制与应用(2011AA10A106);十二五国家支撑计划(2011BAD35B03);四川省青年科技创新研究团队(2014TD0014);现代产业链关键技术创新研究与应用项目(2014NZ0055)共同资助

摘　　要：为明确小麦温敏不育系SCT-1的育性调控基因数量及位点,本研究以组合SCT-1×B2183的F2群体为材料,选用SSR标记和分离群体分组分析法(BSA)筛选与育性相关的分子标记,用完备区间作图法对育性进行初步QTL定位分析。研究结果显示:在2B染色体上存在2个育性调控QTLs:Qwtms-saas-2B-1(位于Xgwm374-Xgwm388间,LOD值4.521)和Qwtms-saas-2B-2(位于Xgwm388-Xbarc101间,LOD值3.115),对表型的贡献率分别为7.649%和13.865%;在5D染色体上检测到1个主效QTL Qwtms-saas-5D-1(位于Xcfd26-Xcfd29间,LOD值11.101),其贡献率达28.093%。研究表明,Qwtms-saas-2B-1与Qwtms-saas-5D-1在成都和新都均能检测到,是育性调控较为可靠的位点。本研究初步定位小麦温敏不育系SCT-1在2B和5D上的育性调控QTLs,可为进一步精确定位奠定基础。In order to make sure the number and locus of fertility genes in thermo-sensitive male sterility wheat line SCT-1, a F2 population derived from the cross SCTv1＆#237;B2183 was studied. The fertility QTLs were mapped by using SSR markers and bulked segregation analysis （BSA）. A genetic linkage map was constructed with ten polymorphism SSR markers by Inclusive Composite Interval Mapping （ICIM） method using the software QTL IciMapping V4.0. Two QTLs designated as Qwtms-saas-2B-1 and Qwtms-saas-2B-2 were detected on chromosome 2B with the LOD value 4.521 and 3.115, respectively. They could explain phenotypic variance 7.649% and 13.865%, separately. One major QTL named as Qwtms-saas-5D-1 was detected on chromosome 5D with the LOD value 11.101, and could explain phenotypic variance 28.093%. The Qwtms-saas-2B-1 and Qwtms-saas-5D-1 could be detected in two experimental stations, Chengdu and Xindu. This work laid the foundations for further study.

关 键 词：温敏不育系 SCT-1 育性基因 SCT-1 

分 类 号：S512.1[农业科学—作物学]