克隆猪隐睾症雌雄激素及其受体基因表达的研究  

Expression of testosterone,estrogen and their receptors in cryptorchidism of cloned pigs

作　　者：李芳芳[1,2,3] 戴建军[1,2] 吴彩凤[1,2] 张树山[1,2] 王昕[1,2,3] 吴鋆龙 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海市农业遗传育种重点实验室动物遗传工程研究室,上海201106 [3]上海海洋大学水产与生命学院,上海201306

出　　处：《上海农业学报》2015年第2期18-21,共4页Acta Agriculturae Shanghai

基　　金：上海市科委农业成果转化专项(123919N0700;133919N1700);国家自然科学基金(31372315);国家转基因生物新品种培育科技重大专项(2009ZX08006-014B;2013ZX08006-005)

摘　　要：利用化学发光免疫技术测定了3头隐睾克隆猪和3头正常猪不同阶段(2—6月龄)的睾酮(T)和雌二醇(E_2)激素的水平;运用相对荧光定量PCR技术检测AR和ER基因在睾丸组织中的mRNA表达量。结果表明:正常猪血清T和E_2浓度从2月龄到6月龄呈上升趋势,隐睾克隆猪血清E_2浓度变化趋势与对照组相似,T变化趋势为先缓慢升高然后降低,5月龄达到最高[(1.98±1.06)ng/mL],且各阶段血清浓度均显著低于正常猪;ER基因在克隆猪睾丸中表达量高于对照组,但差异不显著,AR基因表达量则反之,且差异显著。血清T的低水平和睾丸AR基因mRNA低表达均可能是引起克隆猪发生隐睾的因素。In this study,the serum levels of testosterone（T）and estrogen（E2 ）were detected by fluorescent immunocytometry technology in 3 cloned pigs with cryptorchidism and 3 normal pigs at different stages（2—6 months old）.RT-PCR technique was used to analysis the mRNA expressions of AR and ER gene in testicular tis-sue.The results showed that the serum levels of T and E2 in normal pigs increased with their growth and develop-ment,and the trend of E2 in the cloned pigs was similar with those of normal pigs.The concentration of T in-creased and reached the highest in 5 months old[（1.98 ±1.06）ng/mL],then decreased.Serum levels of Tin cloned pigs were much lower than those of normal pigs.The expression levels of ER gene in cloned pigs were higher than that of normal pigs,but there were no significant differences between them.On the contrary,the ex-pression levels of AR gene in cloned pigs were much lower than those of normal pigs.Results indicated that the lower serum level of T and the abnormal expression of AR gene may be the important factors for the occurrence of cryptorchidism in cloned pigs.

关 键 词：克隆猪 隐睾 睾酮 雌激素 受体 基因表达 

分 类 号：Q78[生物学—分子生物学]