稻瘟病菌MoYpt7互作蛋白的初步筛选  

Primary screening of the interaction proteins of MoYpt7 from Magnaporthe oryzae

作　　者：齐尧尧 周洁[2] 张冬梅[1] 张宏鑫[2] 李光普[1] 鲁国东[1] 

机构地区：[1]福建农林大学植物保护学院 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2015年第2期113-120,共8页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：国家自然科学基金项目(31070124);福建省教育厅重点项目(JA10098)

摘　　要：利用酵母双杂交系统,以稻瘟病菌MoYpt7激活型为诱饵蛋白,从稻瘟病菌不同生长发育阶段的cDNA文库中筛选互作蛋白,经复筛得到10个候选互作蛋白基因,利用Bi FC或pull-down assay验证蛋白间的互作,为寻找MoYpt7下游调控侵染致病过程的效应因子提供依据.Interaction proteins were screened through the yeast two-hybrid system from the cDNA library of different developmental stages of M. oryzae by using the constitutively active version of MoYpt7 as bait protein, and 10 potential interaction proteins genes have been obtained by repeated screening. BiFC or pull-down assay was used to verify the interaction between proteins, so as to offer the evidence for searching the downstream effectors controlling the pathogenicity of M. oryzae.

关 键 词：稻瘟病菌 酵母双杂交 筛选 MoYpt7 互作蛋白 

分 类 号：Q74[生物学—分子生物学]