福州地区豨莶黄脉病病原的分子鉴定  

Molecular identification of the pathogen inducing Siegesbeckia yellow vein disease in Fuzhou

作　　者：张洁[1] 林文武[1] 朱重庆[1] 吴锦鸿[1] 陈晓敏[1] 章松柏[1] 吴祖建[1] 

机构地区：[1]福建农林大学植物病毒研究所/福建省植物病毒学重点实验室,福建福州350002

出　　处：《福建农林大学学报（自然科学版）》2015年第2期126-130,共5页Journal of Fujian Agriculture and Forestry University:Natural Science Edition

基　　金：“十二五”国家科技支撑计划(2012BAD19B03);农业部公益性行业(农业)科研专项(200903034);福建农林大学A类毕业生科研启动基金(132130011)

摘　　要：为明确引起福建省福州地区豨莶上黄脉症状的病原,利用PCR技术,从3个样品上均扩增到双生病毒约500 bp的特异片段,选择病毒分离物Fz02进行全序列测定.结果表明:Fz02 DNA-A全长2771 nts,具有典型的双生病毒的基因组结构特征,与豨莶黄脉病毒广州分离物(Sb YVV-Gz01)同源性最高,达93.4%.利用设计的卫星分子的特异性引物,在病毒分离物Fz02中扩增到betasatellite分子(Fz02β).Fz02β全长1359 nts,与Sb YVV广东分离物(GD13)伴随的betasatellite同源性最高,为85.3%.系统关系树表明,Fz02与Sb YVV各分离物聚类为一个大分支.此外,Sb YVV序列变异较大.To determine the virus species infecting Siegesbeckia orientalis in Fuzhou, Fujian Province, weed samples showing typical geminivirus-like symptoms were tested by PCR using virus-specific primers. A fragment of 500 bp was consistently amplified from to- tal DNA extracts of 3 samples with universal primers PA/PB for geminivirus. Comparison of partial DNA sequences revealed that these samples were infected by a same virus and thus an isolate denoted Fz02 was selected for further sequence analysis. The com- plete sequence of Fz02 comprised 2771 nucleotides with typical genomic organization of begomoviral DNA-A. FzO2 was most closely related to Siegesbeckia yellow vein virus isolate Gz01 （SbYVV-GzO1）, with 93.4% nueleotide sequence identity. Specific primers were designed for the amplification of full length betasatellite genome which was then cloned and sequenced. The complete sequence of betasatellite （FzO2β） was determined to be 1359 nucleotides. Betasatellite shared the highest identity （85.3%） with isolate of ShYVV （ GD13 ）. Phylogenetic analysis showed that Fz02 clustered together with isolates of SbYVV. In addition, there were signifi- cant differences among sequences of SbYVV.

关 键 词：双生病毒 豨莶黄脉病毒 豨莶 序列变异 

分 类 号：S432.1[农业科学—植物病理学]