脑卒中后抑郁大鼠行为学的动态变化及中药补阳还五汤的干预作用  

Dynamic changes of ethology and interventional effect of a traditional Chinese medicine,Buyang Huanwu Decoction,in rats with post-stroke depression

作　　者：罗琳[1] 邓叔华 易健[1] 周赛男[1] 佘颜[1] 刘柏炎[1,2] 

机构地区：[1]湖南中医药大学,长沙410208 [2]益阳医学高等专科学校,益阳413000

出　　处：《中国比较医学杂志》2015年第4期8-13,共6页Chinese Journal of Comparative Medicine

基　　金：国家自然科学基金(编号:81273989);湖南省高校创新平台开放基金项目(12K089);湖南中医药大学病理学与病理生理学重点学科(2012)

摘　　要：目的观察补阳还五汤对脑卒中后抑郁大鼠行为学的影响。方法选择雄性SD大鼠,根据旷场试验基线值,选择评分相近的60只大鼠随机分为假手术组、卒中组、PSD组、补阳还五汤组、氟西汀组,共5组,每组12只。采用大脑中动脉线栓法建立永久性局灶性脑缺血模型结合CUMS及孤养的方法复制PSD模型。通过体重测量、神经功能评分、糖水消耗实验、旷场实验等指标观察各组大鼠行为学变化。结果与假手术组、卒中组比较,PSD组大鼠体重和糖水消耗量降低、旷场实验自发活动下降、神经功能缺损加重(P<0.05或P<0.01);与PSD组比较,补阳还五汤组和氟西汀组均能增加大鼠体重、糖水消耗量及自发行为,改善其神经功能缺损(P<0.05或P<0.01)。结论 PSD大鼠出现行为学异常,补阳还五汤可以明显改善其行为学症状,具有一定抗抑郁作用,其疗效与氟西汀相当。Objective To observe the effect of a traditional Chinese medicine, BuyangHuanwu Decoction, on the ethology in rat models of post-stroke depression （ PSD） .Methods Sixty male SD rats, after open-field ethology scoring, were randomly divided into five groups （ 12 rats in each group）：sham operation group, cerebral ischemic stroke group, post-stroke depression （ PSD） group, BuyangHuanwu Decoction group, and fluoxetine group.The rat PSD models were established with separately breeding and chronic unpredictable moderate stress （ CUMS ） after middle cerebral artery occlusion. Behavior changes of the rats were detected by body weight measurement, neurological scoring, sugar consumption test and open-field test.Results Compared with the sham operation group and cerebral ischemic stroke group, the body weight,sugar consumption and open-field spontaneous activity in the PSD model group were decreased, and the neurological function deficits became more severe （ P〈0.01 or P〈0.05 ） .Compared with the PSD group, Both BuyangHuanwu Decoction group and fluoxetine group got significantly higher scores of body weight,sugar consumption and spontaneous activity,and improved neurological function deficits（P＆lt;0.01 or P＆lt;0.05）.Conclusions PSD rats exhibit abnormal behavior.BuyangHuanwu Decoction can significantly improve the behavioral impairment of PSD rats. The antidepressant effect of BuyangHuanwu Decoction is comparable to that of fluoxetine in the treatment of PSD rats.

关 键 词：补阳还五汤 卒中后抑郁 行为学 大鼠 

分 类 号：R33[医药卫生—人体生理学]