C57-ras转基因小鼠模型杂交1代CB6F1的生长发育特性  

Characterization of growth performance of F1 hybrid CB6F1 C57-ras transgenic mouse model

作　　者：刘甦苏[1] 吴曦[1] 周舒雅[1] 王辰飞[1] 彭泽旭 左琴[1] 李保文[1] 贺争鸣[1] 范昌发[1] 

机构地区：[1]中国食品药品检定研究院,国家啮齿类实验动物种子中心,北京100050

出　　处：《中国比较医学杂志》2015年第4期18-22,共5页Chinese Journal of Comparative Medicine

基　　金：中国食品药品检定研究院中青年发展研究基金(批准号:2010C4)资助的课题

摘　　要：目的测定自主建立的转基因致癌性模型C57-ras小鼠与BALB/c小鼠杂交1代的生长发育特性参数,为该模型的商业化供应提供基础数据。方法将雄性转基因阳性C57-ras小鼠与野生型雌性BALB/c小鼠交配,获得杂交1代(CB6F1 c-Ha-ras+/-)并测定平均产仔数、离乳率、阳性鼠生长发育情况、转基因阳性率等参数。结果在生长发育方面,雌鼠平均每窝产子数为8只,离乳率为90%,雄雌性别比符合预期。CB6F1的平均出生重为(1.73±0.05)g,10周龄时阳性鼠平均体重雌性(24.38±1.74)g,雄性(29.42±1.72)g。雄性鼠体重明显高于雌性,差异存在极显著性(P<0.01);基因型检测阳性率为46.9%,符合遗传规律。结论转基因致癌性模型C57-ras小鼠与BALB/c小鼠杂交1代的生长发育特性正常,可实现规模化商业供应。Objective To obtain the basic growth parameters of a self-established F1 hybrid CB6F1 C57-ras transgenic mouse model, and to provide basic information for commercialization of this mouse model. Methods F1 hybrid mice （CB6F1） were produced by crossing C57-ras heterozygous transgenic （c-Ha-ras＋/-） male mice and wild-type BALB/cJ female mice.The average litter size, weaning rate, sex ratio, growth performance and C57-ras transgenic positive rate were recorded and analyzed.Results The average litter size was eight, weaning rate was 90%, and sex ratio was approximately in accordance with prediction.The average body weight of newborn mice was 1.73 ±0.05 g.The average body weight of 10-week old c-Ha-ras transgenic female and male mice in CB6F1 background was 24.38 ±1.74 g and 29.42 ＆#177;1.72g, respectively, which had a significant difference （P〈0.01）.The c-Ha-ras transgenic positive rate was 46.9%. which was in accordance with genetic rules.Conclusions The F1 hybrid mice （CB6F1） produced by crossing C57-ras heterozygous transgenic （ c-Ha-ras ＋/-） male mice and wild-type BALB/cJ female mice show normal growth performance and development characteristics, and it can be used for large-scale commercial supply.

关 键 词：C57-ras转基因小鼠 人原癌基因c-Ha-ras CB6F1 生长发育特性 

分 类 号：R33[医药卫生—人体生理学]