17β雌二醇对大鼠创伤性脑损伤的保护作用  

Protective effect of 17β-estradiol on traumatic brain injury in rats

作　　者：王迅[1] 冷晓磊[1] 矫永庆[1] 崔鹏[2] 

机构地区：[1]大连市第三人民医院神经外科,辽宁大连116033 [2]大连医科大学附属三院神经外科,辽宁大连116000

出　　处：《中国比较医学杂志》2015年第4期23-26,共4页Chinese Journal of Comparative Medicine

摘　　要：目的探讨大鼠创伤性脑损伤后17β雌二醇对脑组织的保护作用。方法选择雄性成年SD大鼠45只,按随机数字表法分为三组,每组15只:对照组仅开骨窗,不损伤脑组织;致伤组制大鼠自由落体脑撞击伤模型;处理组在致伤组的基础上,伤前1周腹腔注射17β雌二醇溶液(1 mg/kg),1次/d。余两组仅注射同体积的蓖麻油。在伤后6 h、24 h及48 h测量各组脑组织含水量、丙二醛(MDA)含量及超氧化物歧化酶(SOD)活性。结果致伤组和处理组在伤后6 h、24 h、48 h三个时间点脑组织含水量均高于对照组(P<0.05)。在伤后6 h,致伤组和处理组脑组织含水量比较差异无统计学意义[(58.39±0.29)%比(57.03±0.27)%](P>0.05),而在伤后24h、48 h致伤组脑组织含水量明显高于处理组,差异有统计学意义[(67.41±0.37)%比(64.77±0.33)%,(81.95±0.47)%比(75.26±0.41)%](P<0.05)。在伤后6 h致伤组、处理组MDA含量明显增加,并且一直维持在较高水平,而SOD活性则明显下降,与对照组比较差异有统计学意义(P<0.05)。处理组在伤后6 h MDA含量和SOD活性与致伤组比较差异无统计学意义(P>0.05),而在伤后24 h、48 h MDA含量显著降低[(130.39±7.02)μmol/g比(149.41±8.25)μmol/g,(125.41±6.59)μmol/g比(157.72±8.93)μmol/g],SOD活性则明显增高[(88.46±7.17)U/g比(80.10±4.87)U/g,(97.31±7.89)U/g比(84.29±6.13)U/g],差异均有统计学意义(P<0.05)。结论 17β雌二醇对创伤性脑损伤有保护作用。Objective To explore the protective effect of 17β-estradiol on traumatic brain injury in rats. Methods A total of 45 adult male SD rats were divided into 3 groups using the random digit table, 15 rats in each group： the control group only exposed but not injured the brain, the injury group received traumatic brain injury （TBI） by Feeney＇ s method, and the treatment group received the same handling with injury group, and pretreatment with 17β-estradiol peritoneal injection, I mg/kg per day for one week. The other two groups were given the same volume of castor oil. At 6 h, 24 h and 48 h after injury, the brain tissue water content, malondialdehyde （MDA） content and superoxide dismutase （SOD） activity were measured. Results At 6 h, 24 h and 48 h after injury, the levels of brain tissue water content in the injury group and treatment group were significantly higher than those in the control group （ P 〈 0. 05 ）. At 6 h after injury, the brain tissue water contents in the injury group and treatment group were （99. 83 ±0.40） % and （99.53 ±0. 41 ） % ,respectively, with a non-significant difference between the two groups （ P 〉 0.05）. At 24 h and 48 h after injury, the brain tissue water contents in the injury group was （105. 17 ± 0.43 ）% and （107.54 ±0.39）% , in the treatment group was （ 103.26 ± 0. 42） % and （ 105.89 ±0. 43 ） % , respectively, showing a significant ditterence between the two groups （ P 〈 0.05 ）. At 6 h after injury, the levels of MDA in the injury group and treatment group increased and maintained at a higher level, and the levels of SOD decreased. Compared with the control group, there had a significant difference （ P 〈 0.05 ）. The levels of MDA and SOD in the injury group and treatment group at 6 h after injury had a non-significant difference （ P 〉 0.05 ）. But at 24 h and 48 h after injury, the levels of MDA in the treatment group were significantly lower than those in the injury group [（130.39±7.02） μmol/g vs. （14

关 键 词：雌二醇 脑损伤 丙二醛 超氧化物歧化酶 脑组织含水量 大鼠 

分 类 号：R33[医药卫生—人体生理学]