蕨麻小型猪与巴马小型猪高原多脏器功能障碍综合征模型的比较研究  

Comparison of the Bama minipig and Juema minipig models of high altitude multi-organ dysfunction syndrome

作　　者：牛廷献[1] 郭晓宇[1] 申健[1] 王红义[1] 陆璐[1] 肖攀[1] 冯小明[1] 

机构地区：[1]兰州军区兰州总医院,兰州730050

出　　处：《中国比较医学杂志》2015年第4期33-37,F0002,共6页Chinese Journal of Comparative Medicine

基　　金：甘肃省技术研究与开发专项计划(1205TCYA010)

摘　　要：目的比较不同海拔地区两种小型猪高原多脏器功能障碍综合征(H-MODS)模型存在的差异,探讨建立H-MODS模型的理想动物。方法利用高原土生蕨麻小型猪与平原巴马小型猪,以内毒素(LPS)建立HMODS模型。检测不同时点血液生理生化指标的变化,并观察肺脏组织病理学变化。结果巴马小型猪模型的死亡率明显高于蕨麻小型猪;生理生化各指标的变化基本相同,但蕨麻小型猪的变化较平缓。在24 h与48 h肺脏均出现严重病理改变,但巴马小型猪较蕨麻小型猪更严重。结论不同海拔地区的两种小型猪均可成功建立HMODS模型,同时蕨麻小型猪由于自身高原生物特性对H-MODS模型的复制更加准确安全,避免高原环境的外在因素的影响。Objective To compare the Bama minipig and Juema minipig models of high altitude multi-organ dysfunction syndrome.Methods Six plateau-origin Juema minipigs and plain-origin Bama minpigs in each group received intravenous infusion of 0.35 mg/kg lipopolysaccharide （ LPS） , respectively.Blood samples were taken at 0 h, 3 h, 6 h, 12 h, 24 h, 48 h and 72 h after LPS infusion.Routine blood test was performed, blood CK, AST, ALT, TBIL, CRE were assayed, and histopathological examination of the lung tissues was performed at 24 h, 48 h after LPS infusion.Results The mortality of Bama minipigs was 33.3%, higher than that of 16.7%of Juema minipigs.The trend of physiological and biochemical changes was similar, but was milder in the Juema minipigs than in Bama minipigs.The lung injuries of the Bama minipigs at 24 h and 48 h were more severe than those in the Juema minipigs.Conclusions Both Bama and Juema minipig models of high altitude multi-organ dysfunction syndrome can be successfully established.Juema minipig models can be more closely and safely established, due to its own plateau biological properties, and avoid the influence by extrinsic injurious effects of plateau environment.

关 键 词：高原多脏器功能障碍综合征模型 蕨麻小型猪 巴马小型猪 比较 

分 类 号：R33[医药卫生—人体生理学]