中药藤莓汤对Ⅱ型胶原诱导性大鼠关节炎性病理损伤的影响  

Effects of the Chinese herbal compound,Tengmei decoction,on inflammatory injury in joints of rat models of type Ⅱcollagen-induced arthritis

作　　者：陆妍[1] 王亚南[1] 刘慧[1] 马卫国[1] 张继胜[1] 谭玲 李慧慧 林子超[1] 沈志明[1] 王洁[1] 孟凤仙[1] 

机构地区：[1]北京中医药大学东方医院风湿科,北京100078 [2]昌平区中西医结合医院脑病科,北京102208 [3]密云县中医院风湿科,北京101500

出　　处：《中国比较医学杂志》2015年第4期48-57,I0004,共11页Chinese Journal of Comparative Medicine

摘　　要：目的探讨中药藤莓汤对Ⅱ型胶原诱导性关节炎(collagen-induced arthritis,CIA)大鼠关节滑膜炎症指数、四肢肿胀程度、关节组织炎性浸润的改善情况,以及免疫炎性相关因子影响。方法建立CIA模型,将造模成功的SD大鼠随机分为模型组、阳性药组、中药大、小剂量组,设正常对照组,每组6只,干预治疗12周。每周使用游标卡尺测量四肢踝腕部前后径、左右径,爪掌足垫厚度,以及双后肢的跖关节肿胀最高点宽度,评价其关节积分和四肢肿胀程度。结果 1与正常组比较,模型组大鼠关节炎总AI评分及双后肢AI评分显著增加(P<0.05或P<0.01);左前肢AI评分在10-12周时升高(P<0.05);左后肢前后径、左右径、足垫厚度及左后肢跖骨最高点测量值在1-12 w不同时间段增加(P<0.05或P<0.01)。与模型组比较,中药大、小剂量组总AI评分及左后肢关节AI评分在6 w后降低(P<0.05);阳性药组10-12 w时左前肢AI评分下降(P<0.05)。2与正常组比较,模型组IL-6、TNF-αmRNA转录及蛋白表达水平显著上调(P<0.01)。与模型组比较,阳性药组、中药大、小剂量组IL-6、TNF-αmRNA转录及蛋白表达水平显著下调(P<0.01)。3光镜检测结果显示:中药小剂量组明显改善了CIA模型大鼠关节滑膜增生、炎性细胞浸润情况。结论中药藤莓汤改善CIA模型大鼠关节病理损伤的分子机制可能与其抑制滑膜组织免疫炎性因子高表达相关。Objective To explore the effects of Chinese herbal compound, Tengmei decoction, on type II collagen-induced arthritis （ CIA） in rats, and to examine the changes of arthritis index （ AI） , limb swelling, joint tissue inflammatory infiltration, and the effects on immune-inflammatory factors.Methods Sprague-Dawley rat models of arthritis were successfully established by intradermal injection of type II collagen and Freund’ s complete adjuvant.The model rats were randomly divided into model group, positive drug group, and high-and low-dose Chinese medicine groups, 6 rats in each group.The intervention and treatment period was 12 weeks.To measure weekly the anteroposterior and transverse diameters of the rear ankles and wrists, the transverse diameter of the claw foot palm pad, the thickness and the highest point width of hind limb plantar joint swelling, and to evaluate the integrated scores of joints and limbs swelling using a vernier caliper.Results ①Compared with the normal group, the total arthritis scores and hind limbs AI scores of the model group were significantly increased （ P 〈0.05 or P 〈0.01 ） .The left forelimb AI scores were significantly increased during 10 -12 weeks （ P 〈0.05 ） .The anteroposterior and transverse diameters of the left hind limb, the thickness of the highest point measurement of the left hind foot pad metatarsal were significantly increased （ P〈0.05 or P〈0.01） in different time periods between 1-12 weeks.Compared with the model group, the total scores and the left hind limb joints AI scores of the high-and low-dose drug groups were decreased after 6 weeks （P〈0.05）.②Compared with the normal control group, levels of mRNA transcription and protein expression of IL-6 and TNF-αwere significantly up-regulated （ P〈0.01 ） in the model group.Compared with the model group, the levels of mRNA transcription and the expression of IL-6 and TNF-αproteins were significantly down-regulated in the positive group and Chinese medicine groups （ P 〈0.01 �

关 键 词：中药藤莓汤 CIA模型 AI积分 病理改变 免疫炎性机制 

分 类 号：R33[医药卫生—人体生理学]