食蟹猴脐带间充质干细胞的分离与鉴定  

Isolation and identification of cynomolgus monkey umbilical cord mesenchymal stem cells

作　　者：庞荣清[1] 何洁[1] 李瑞生[2] 赵晶[1] 朱慧[1,3] 朱向情[1] 阮光萍[1] 潘兴华[1] 

机构地区：[1]成都军区昆明总医院云南省干细胞工程实验室,昆明650032 [2]解放军第302医院实验技术研究保障中心,北京100039 [3]昆明医科大学昆明总医院临床学院,昆明650031

出　　处：《中国比较医学杂志》2015年第4期66-69,F0003,共5页Chinese Journal of Comparative Medicine

基　　金：云南省自然基金项目(2011HB050;2013DA004)

摘　　要：目的建立食蟹猴脐带间充质干细胞的分离培养方法。方法新鲜食蟹猴脐带剪碎为糊状,用含10%胎牛血清的DMEM/F12培养液培养,观察细胞形态特征,流式细胞技术分析细胞抗原标志的表达,并检测其体外多向分化潜能。结果运用组织贴块法可以从新鲜脐带中分离到贴壁生长、阳性表达CD29、CD44、CD90的成纤维细胞样细胞。这些细胞在体外诱导培养后可分别检测到脂滴、骨和软骨细胞。结论运用组织贴块培养法可用含10%胎牛血清的DMEM/F12培养液从食蟹猴脐带中分离到间充质干细胞。Objective To establish a method for isolation of cynomolgus monkey umbilical cord mesenchymal stem cells.Methods Fresh cynomolgus monkey umbilical cord was directly minced into pasty fine pieces, and the pieces were cultured in tissue flask with DMEM/F12 medium supplemented with 10% fetal bovine serum.The morphological characteristics of the resulting cells were examined, and their expression of mesenchymal cell surface markers were analyzed by flow cytometry.The multidifferentiation potential was examined in vitro, too.Results The fibroblast-like cells were successfully isolated from the fresh umbilical cord by an adherent culture procedure.These adherent cells expressed mesenchymal markers including CD29, CD44, and CD90, and also could be induced to differentiate into adipocytes, osteoblasts and chondrocytes.Conclusion Mesenchymal stem cells can be isolated from fresh cynomolgus monkey umbilical cord by using an adherent culture procedure.

关 键 词：脐带间充质干细胞 食蟹猴 培养方法 

分 类 号：R33[医药卫生—人体生理学]