基于线粒体COⅠ～COⅡ序列的黑带食蚜蝇(Episyrphus balteatus)遗传多样性初探  

Genetic Diversity of Episyrphus balteatus Based on Mitochondrial DNA Sequence COⅠ～COⅡ

作　　者：张卓[1] 胡建楠 王晓昕[1] 周婵[2] 刘广纯[1] 

机构地区：[1]沈阳大学城市有害生物治理与生态安全重点实验室,辽宁沈阳110044 [2]辽宁大学生命科学院,辽宁沈阳110036

出　　处：《沈阳大学学报（自然科学版）》2015年第2期116-119,共4页Journal of Shenyang University：Natural Science

基　　金：国家自然科学基金资助项目(31071965;81102296);2014年辽宁省创新创业项目(201411035000030);沈阳大学博士启动基金资助项目(1120212341)

摘　　要：利用COⅠ的通用引物,采用PCR法对黑带食蚜蝇(Episyrphus balteatus)线粒体DNA序列进行扩增、测序及比对;4个不同地区的种群分析结果表明:1共获得1041个bp的DNA序列,A、T、G、C的比例分别为40.2%、30.1%、13.1%、16.6%,表现出明显的A+T偏倚性;2经过与GENEBANK已知序列比对,该片断位于线粒体的COⅠ~tRNALEU^COⅡ区域;3共检测到8处(N=20)碱基变异,已经产生了遗传差异,形成1个共有单倍型、7个特有单倍型;4NETWORK分析表明,已经产生明显的遗传分化.Mitochondrial DNA of F.pisyrphus balteatus population from four areas were amplified, sequenced and compared with CO I universal primer. The results show that there was 1041bp in DNA sequences of four populations. The percentage of A, T, G, C were 40. 2%, 30. 1%, 13. 1%, and 16.6%, respectively. And DNA sequences showed obvious bias on A＋T. Sequence alignment in GENEBANK showed that DNA sequences was located in the mitochondrial CO I -tRNALEU-CO Ⅱ region. Eight bases had variations, which indicated that population had genetic differences with one common haplotype and seven special haplotypes. had significant genetic differentiation.

关 键 词：黑带食蚜蝇 遗传多样性 COⅠ~COⅡ 种群 生物地理 

分 类 号：Q95[生物学—动物学]