HPLC-DAD法同时测定“武当二号”忍冬叶中绿原酸和木犀草苷的含量  

Simultaneous Determination of Chlorogenic Acid and Luteoloside in the Leaves of “Wudang No.Ⅱ” Flos Lonicerae by HPLC-DAD

作　　者：李鹏[1] 郑芳[1] 李聪[1] 李志浩[1] 黄麟杰[1] 朱雪松[1] 

机构地区：[1]湖北医药学院附属东风医院药学部武当特色中药研究湖北省重点实验室,十堰442008

出　　处：《医药导报》2015年第5期660-663,共4页Herald of Medicine

基　　金：湖北省2011协同创新中心基金(4)

摘　　要：目的建立同时测定＂武当二号＂忍冬叶中绿原酸和木犀草苷含量高效液相色谱测定方法。方法采用Phenomenex C18（4.6 mm×250 mm,5μm）色谱柱;流动相为乙腈（A）-0.4%磷酸溶液（B）进行梯度洗脱;检测波长350 nm;流速0.8 m L·min^-1;柱温32℃。结果绿原酸、木犀草苷分别在进样量0.285~2.280,0.124~1.240μg范围内与峰面积呈良好的线性关系,相关系数分别为0.999 3,0.999 4;平均加样回收率分别为98.9%,98.8%（n=6）,RSD分别为1.59%,1.84%。结论该方法准确、快速、重复性好,可以用来同时测定＂武当二号＂忍冬叶中绿原酸和木犀草苷的含量。Objective To establish a HPLC method for simultaneous determination of chlorogenic acid and luteoloside in the leaves of“Wudang No.II” flos lonicerae. Methods Phenomenex C18（4. 6 mm×250 mm, 5μm） was used;the mobile phase was acetonitrile（ A） and 0. 4% phosphoric acid aqueous solution（ B） by gradient elution mode; the detection wavelength was 350 nm and the flow rate was 0. 8 mL·min^-1;the column temperature was set at 32℃. Results The calibration curve of chlorogenic acid and luteoloside was linear in the range of 0. 285-2. 280μg（r=0. 999 3）, and 0. 124-1. 240μg（r=0. 999 4）, respectively. The mean recovery of chlorogenic acid and luteoloside was 98. 9%, RSD=1. 59% and 98. 8%, RSD=1. 84%, respectively. Conclusion This method was found to be accurate, quick and reproducible. It can be used for simultaneous determination of chlorogenic acid and luteoloside in the leaves of “Wudang NO.II”flos lonicerae.

关 键 词：“武当二号”忍冬叶 绿原酸 木犀草苷 检测器 高效液相色谱法-二极管阵列 

分 类 号：R282.71[医药卫生—中药学] R927.2[医药卫生—中医学]