微生物转化氧化槐定碱活性菌株的筛选  

Strains screening on microbial transformation of oxysophoridine

作　　者：付少彬[1] 文福来[1] 闫松[1] 张芸[1] 李义[1] 岳昌武[2] 

机构地区：[1]遵义医学院药学院,贵州遵义563099 [2]遵义医学院医学与生物学研究中心暨贵州省特色微生物资源及药物开发重点实验室,贵州遵义563099

出　　处：《遵义医学院学报》2015年第2期190-192,196,共4页Journal of Zunyi Medical University

基　　金：国家自然科学基金资助项目(NO:21462057);贵州省普通高等学校微生物资源及药物开发特色重点实验室开放基金(NO:GZMRD-2014-003);贵州省中医药管理局中医药;民族医药科学技术课题研究资助(NO:QZYY-2014-083);大学生创新创业项目(NO:[2014]5838)

摘　　要：目的通过微生物转化方法对氧化槐定碱进行结构修饰和改造,以期得到结构新颖或活性更强的衍生物,从中筛选出对氧化槐定碱有催化活性的微生物菌株。方法采用稀释法分离土壤微生物菌株,表面消毒法分离植物内生菌,通过薄层色谱法(TLC)对转化反应进行筛选;通过表型和基因型鉴定活性菌株。结果从遵义医学院土壤分离得到土壤微生物菌株26株,从植物分离得到内生真菌60株;17株土壤微生物具有催化活性,其中菌株T003表现出强的选择性,经显微结构和16S r DNA序列分析,鉴定其为Escherichia coli。结论微生物转化法为氧化槐定碱的结构修饰和改造提供了一个很好的选择方法。Objective The structuture of oxysophoridine （ORS） were modified by microbial transformation to ob- tain derivatives with novel structure or Stronger activity. The strains with catalytic activity of oxysophoridine were screened. Methods Soil microbes were separated and purified by dilution method while plant endophytic fungi were obtained by surface disinfection. Transformation reaction was detected via TLC. Active stains were identi- fied by phenotype and genotype. Results Twenty - six soil microbes were isolated from soil in Zunyi Medical Uni- versity and 60 endophytic fungi from plant were purified. Seventeen strains of soil microbes exhibited the catalytic activity. Interestingly, strain T003 with high selectivity was identified as Escherichia coli based on phenotype and genotype. Conclusion It provides an alternative for structure modification of ORS by microbial transformation.

关 键 词：生物转化 生物碱 土壤微生物 选择性 

分 类 号：R284[医药卫生—中药学]