D3卵裂期胚胎玻璃化冷冻技术在IVF实验室中的应用  

Application of D3 cleavage stage embryo vitrification freezing technology in IVF laboratories

作　　者：葛斌[1] 杨智敏[1] 刁英[1] 杨名慧[1] 宫黎明[1] 石燕[1] 

机构地区：[1]遵义医学院附属医院生殖中心,贵州遵义563099

出　　处：《遵义医学院学报》2015年第2期193-196,共4页Journal of Zunyi Medical University

基　　金：遵义医学院硕士启动基金资助项目(NO:2007-54)

摘　　要：目的探讨玻璃化冷冻技术在人类卵裂期胚胎冻存中的应用价值。方法回顾性分析本中心261个冷冻胚胎复苏周期,比较2种冷冻方法的胚胎存活率、完好率、妊娠率和种植率等数据。结果玻璃化冷冻组的存活率(97.11%)、完好率(89.02%)和种植率(23.12%)显著高于程序化组(91.31%、63.73%、16.31%)(P<0.05),而妊娠率和平均年龄、平均移植胚胎数、平均内膜厚度两组间无统计学差异(40.26%vs40.22%、31.3±5.3vs32.2±5.5、2.25±0.55vs2.27±0.70、8.9±1.1vs9.1±1.6)(P>0.05)。结论玻璃化冷冻是卵裂期胚胎保存的优选方法。Objective To explore the development potential of embryo and its breadboard outcome after cryopr- eservation with vitrification and programmed freezing methods. Methods In the retrospective study, a total of 261 frozen embryos were studied. The ratio of embryos survival, blastomere integrity, implantation and clinical preg- nancy were compared between these two methods. Results Compared with the programmed frozen embryos, the Vitrified embryos had a higher survival rate （97.11% vs 91.31% ）, blastomere integrity rate （89.02% vs 63 73% ） and implantation rate （23.12% vs 16.31% ） （P 〈0.05）. However, there was no significant difference in clinical pregnancy rate（40.26% vs 40.21% ） , the average number of transferred embryos （2.25±0.55 vs 2.27 ± 0.70）, the average age （31.3 ± 5.3 vs 32.2 ± 5.5 ） and the endometrial thickness （8.9 ± 1.1 vs 9.1 ± 1.6） between these two different freezing methods （P 〉 0.05 ）. Conclusion The outcome of vitrification is better than the programmed freezing method.

关 键 词：卵裂期胚胎 玻璃化更冷冻 程序化冷冻 

分 类 号：R321.3[医药卫生—人体解剖和组织胚胎学]