早产儿氧暴露后外周血单个核细胞内活性氧簇产生与自噬变化的关系  

The relationship between reactive oxygen species and autophagy within peripheral blood mononuclear cells in premature infants after oxygen exposed

作　　者：苏剑 董文斌 李清平 康兰 张莲玉 卢佑英 翟雪松 

机构地区：[1]四川医科大学附属医院新生儿科,四川泸州64600

出　　处：《西部医学》2015年第5期656-659,共4页Medical Journal of West China

基　　金：四川省教育厅科研课题(08ZA150);四川省卫生厅科研课题(90191)

摘　　要：目的观察早产儿氧暴露后外周血单个核细胞内活性氧簇产生与自噬的水平,探讨活性氧簇产生与自噬变化的关系.方法32周以下早产儿根据用氧浓度不同分为三组,FiO2＜ 30％为低浓度吸氧组、FiO2在30％～40％为中浓度吸氧组、FiO2＞ 40％为高浓度吸氧组.各组氧疗48小后,与对照组中同期未吸氧的32周以下早产儿,经桡动脉采血3ml,分离外周血单个核细胞（PBMCs）及血清,采用激光共聚焦显微镜观察检测细胞内活性氧（ROS）的生成量,硫代巴比妥酸比色法检测血清丙二醛（MDA）含量,丹（磺）酰戊二胺（MDC）结合激光共聚焦显微镜观察自噬表达水平.结果早产儿氧暴露后,随着用氧浓度的升高,PBMCs内ROS.与其余三组相比,高浓度吸氧组中ROS,MDA含量和自噬体表达逐渐增高明显增加（P＜0.05）,各组ROS水平与自噬表达水平呈正相关（P＜0.01）.结论早产儿氧暴露后,高氧可诱导体内ROS增多,并在细胞内出现自噬现象.ROS可作为信号分子引起和上调细胞发生自噬反应。Objective To explore the relationship between reactive oxygen species and autophagy within peripheral blood mononuclear cells in premature infants after oxygen exposed. Methods According to different oxygen concentra- tions, the preterm infants were divided into three groups including low concentration oxygen group （FiO2 〈30 %）, mid- dle concentration oxygen group （FiO2 between30- 40%） and high concentration oxygen group （FiO2 〉40%）. Premature infants less than 32 weeks without oxygen were as control group. After 48h, 1 blood were taken via radial artery and used to separate peripheral blood mononuclear cells（PBMCs）and serum. The intracellular reactive oxygen species （ROS） were detected by confoeal laser scanning microscopy. The level of malondialdehyde （MDA）in serum were measured by thiohar- bituric acid colorimetric. The expression of autophagy within peripheral blood mononuclear cells were observed by mono- dansylcadaverin combined with confocal laser scanningmicroscope. Results After premature infants exposed to oxygen, the oxygen, ROS and expression of autophagy and MDA were gradually increased. Compared that of the other three groups, the level of ROS, expression of autophagy and MDA in the high concentration oxygen group were increased sig- nificantly （P〈0.05）. There were positive correlations of autophagy and ROS in PBMCs among the groups（P〈0.01）. Conclusion High oxygen could induce in ROS increased in premature infants. The emergence of autophagy ROS could be used as a signal molecule induced upregulation of autophagy and cell reaction.

关 键 词：早产儿 活性氧 自噬 

分 类 号：R722.6[医药卫生—儿科]