子痫前期患者子宫蜕膜自然杀伤细胞的表型研究  

Study on phenotype of decidual NK cells in preeclampsia patients

作　　者：姜妮娜 

机构地区：[1]青岛市胶州中心医院,山东青岛266300

出　　处：《西部医学》2015年第5期745-747,750,共4页Medical Journal of West China

摘　　要：目的观察先兆子痫患者子宫蜕膜自然杀伤细胞(dNK细胞)的表型。方法选取2012年12月至2013年5月在我院产科住院的30例子痫前期患者作为病例组,随机选取同期在我院产前检查正常的晚期妊娠妇女30例作为正常对照组。收集孕晚期子宫蜕膜组织,机械研磨加梯度离心法提取蜕膜内单核细胞,流式细胞技术(FCM)筛选出dNK细胞,并检测dNK细胞表面CD56及CD16的表达情况。结果病例组与正常对照组CD56bright CD16-CD3-dNK细胞的数量均明显多于CD56dim CD16+CD3-dNK细胞(P<0.05),病例组与正常对照CD56bright CD16-CD3-dNK细胞所占比例无显著差异(P>0.05),CD56dim CD16+CD3-dNK细胞所占比例也无显著差异(P>0.05)。结论先兆子痫患者与正常孕妇孕晚期子宫蜕膜内dNK细胞表型没有明显不同,均以CD56bright CD16-CD3-亚型为主。Objective To investigate the phenotype of uterine decidual natural killer cells（dNK cells） in preeclamp- sia patients. Methods 30 women with preeclampsia were selected as case group. 30 normal pregnant women were select- ed as normal control group. The decidual tissues were sampled immediately after caesarean section. The mononuclear cells were extracted from the tissues by means of mechanic grinding and gradient centrifugation. The technique of flow cytometry was used for dNK cell sorting and the expression of CD56 and CD16 on the surface of cells was also examined. Results In case group and normal control group, the proportions of CD56bright CD16- CD3- dNK cells were significantly higher than those of CD56dim CD16＋ CD3- dNK cells（P〈0.05）. Neither the CD56bright CD16- CD3- subtype nor the CD56dim CD16＋ CD3- subtype had statistical difference between case group and normal control group（P〉0.05）. Conclu- sion The phenotypes of dNK cells from the women with preeclampsia and from healthy pregnant women are both domi- nated by CD56bright CD16- CD3- subtype, without significant difference.

关 键 词：子痫前期 子宫蜕膜 自然杀伤细胞 细胞表型 母-胎界面 

分 类 号：R714.245[医药卫生—妇产科学]