白芍药材中微量元素与有效成分含量相关性分析及灰色关联度评价  

Study on the relationship between trace elements and active ingredients in Radix Paeoniae Alba and evaluation of grey indidence degree

作　　者：刘威[1,2] 王金玲[2] 胡军华[2] 李家春[2] 张振秋[1] 萧伟[2] 

机构地区：[1]辽宁中医药大学药学院,大连116600 [2]江苏康缘药业股份有限公司博士后工作站,连云港222001

出　　处：《中华中医药杂志》2015年第5期1400-1406,共7页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家"重大新药创制"科技重大专项(No.2009ZX09504-004)~~

摘　　要：目的:通过测定不同产地白芍药材中没食子酸、芍药苷等7个成分及Fe、Mg等24种微量元素的含量,探讨白芍药材中物质成分间的关系,为白芍药材的质量评价提供参考。方法:采用微波消解-电感等离子体质谱法(ICP-MS)测定白芍中24种微量元素的含量;采用高效液相色谱法(HPLC)测定没食子酸、芍药内酯苷、芍药苷、1,2,3,4,6-五没食子酰葡萄糖、苯甲酸、苯甲酰芍药苷、丹皮酚7个成分的含量;通过灰色关联和相关分析法对不同产地白芍的质量排序及主成分含量和微量元素的相互关系进行分析。结果:不同产地白芍主成分及微量元素含量有一定的差异,白芍中的不同成分、各种微量元素之间存在一定的相关性。结论:上述方法及模型可以用于白芍药材质量评价。Objective： Determination of the contents of 24 trace elements and 7 components in the medicinal plant Radix Paeoniae Alba which grew the different areas, to discuss the relationships between its various constituents, so as to provide a reference for the quality control of Radix Paeoniae Alba. Methods： Microwave digestion-inductively coupled plasma mass spectrometry （ICP-MS） was used for the determination of the contents of trace elements and high performance liquid chromatography （HPLC） for the determination of the contents of gallic acid, albiflorin, paeoniflorin, 1,2,3,4,6-penta-o-galloyl- beta-d-glucopyranose, benzoic acid, benzoyl paeoniflorin, paeonol. Grey incidence degree and correlation analysis method were employed to sort the quality of Radix Paeoniae Alba from the different areas and analyze the relationship between the contents of main components and trace elements. Results： There were certain differences and correlations between the principal components and the content of trace elements in Radix Paeoniae Alba from different areas. Conclusion： Grey incidence degree method, correlation analysis method, and the models could evaluate the quality of Radix Paeoniae Alba.

关 键 词：白芍 微量元素 有效成分 灰色关联 相关分析 

分 类 号：R284[医药卫生—中药学]