川芎嗪对白介素-1β诱导退变的椎间盘终板软骨细胞的保护作用  

Protective effect of ligustrazine on degenerated endplate chondrocytes in intervertebral disc induced by interleukin-1β

作　　者：陈岩[1,2] 王拥军[1,2] 徐浩[1,2] 丁道芳[1,2] 周泉[1,2] 卢盛[1,2] 施杞[1,2] 梁倩倩[1,2] 

机构地区：[1]上海中医药大学附属龙华医院,上海200032 [2]上海中医药大学脊柱病研究所,上海200032

出　　处：《中华中医药杂志》2015年第5期1437-1442,共6页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家教育部"创新团队发展计划"(No.1RT1270);国家自然科学基金重点项目(No.81330085);国家自然科学基金重大国际合作项目(No.81220108027);国家自然科学基金面上项目(No.81173278);国家自然科学基金青年科学基金项目(No.81403417;No.81403418);全国优秀博士学位论文作者专项资助(No.201276)~~

摘　　要：目的:研究川芎嗪对白介素-1β(IL-1β)诱导退变的终板软骨细胞的影响。方法:体外分离培养大鼠椎间盘终板软骨细胞,并用IL-1β和川芎嗪处理,进行细胞增殖、细胞免疫化学染色和RT-PCR检测。结果:川芎嗪(6.25-25.00μmol/L)逆转IL-1β对大鼠终板软骨细胞增殖的抑制影响,抑制IL-1β诱导的软骨细胞IL-1β、肿瘤坏死因子-α(TNF-α)、环氧化酶-2(COX-2)和诱导型一氧化氮合酶(i NOS m RNA)过量表达,逆转IL-1β对于Col2-1、Aggrecan、MMP-13和ADAMTS-5 m RNA表达的影响,增加IL-1β诱导后Ⅱ型胶原的蛋白表达。结论:本研究证实川芎嗪可以抑制IL-1β诱导后软骨细胞的退变表现。Objective： To observe the protective effect of ligustrazine on degenerated endplate chondrocytes induced by interleukin-1β （IL-1β ）. Methods： Rat endplate cbondrocytes were isolated and cultured with IL-1β （10ng/mL） and ligustrazine （0-25 μmol/L） in vitro. The tests of cell proliferation, cell immunochemical staining and RT-PCR were processed. Results： Ligustrazine （6.25-25.00μm/L） could reverse the proliferation inhibition influence of rat endplate cartilage cell caused by IL-113, inhibit over expression of IL-1β, TNF-α, COX-2 and iNOS mRNA induced by IL-1 β, reverse the influence on expression of Co12-1, Aggrecan, MMP-13 and ADAMTS-5 mRNA caused by IL-1 β, and increase typeⅡ collagen protein expression after abduction of IL- 1 β. Conclusion： Ligustrazine could inhibit the degeneration performance of chondrocyte after abduction of IL- 1β.

关 键 词：川芎嗪 白介素-1Β 软骨细胞 Ⅱ型胶原 基质金属蛋白酶-13 

分 类 号：R285[医药卫生—中药学]