不同类型猴艾滋病模型进展因素探讨  

Exploring the progressive factors of different simian immunodeficiency virus-infected rhesus macaque models

作　　者：张苗苗[1] 陈剑涛[1] 成业[1] 朱伯强 周红燕[1] 符林春[1] 

机构地区：[1]广州中医药大学热带医学研究所艾滋病研究室,广州510405

出　　处：《中华中医药杂志》2015年第5期1542-1545,共4页China Journal of Traditional Chinese Medicine and Pharmacy

基　　金：国家自然科学基金(No.81302897;No.81173436)~~

摘　　要：目的:探讨健康恒河猴(正常组)和已感染猴免疫缺陷病毒(SIV)的长期不进展(LTNP组)、普通进展(NP组)、快速进展(RP组)恒河猴模型在感染前后的差异。方法:挑选符合LTNP、NP、RP特点的3种类型恒河猴各10只,与同期5只健康恒河猴对比,分析感染前后不同类型间T细胞亚群、中医证候指标及淋巴结病理的不同之处。结果:各组动物血浆病毒载量均在感染后10-14d达到高峰,平台期出现分化。与正常组相比,RP组的WBC明显偏高,CD4和LYM比例明显偏低;而LTNP组的LYM数量偏高;与其它类型相比,RP组的β2-MG显著升高,T3显著降低。RP型在平台期出现淋巴结缩小,生发中心明显缩小的退变型,区别于其它各型。结论:LTNP、NP和RP 3种进展类型在平台期有明显差异,其中RP型有更明显的脾肾俱虚证候,提示脾肾俱虚有可能是艾滋病快速进展的关键因素。Objective： To explore the difference among healthy rhesus and rhesus infected with simian immunodeficiency virus （SIV） in long-term non-progress （LTNP）, normal progress （NP）, rapid progress （RP）. Methods： Rhesus infected with LTNP immunodeficiency virus, NP immunodeficiency virus and RP immunodeficiency virus were selected, and with 10 rhesus of each kind, at the same time, 5 healthy rhesus were compared. To analyze the difference in T cell subset, traditional Chinese medicine syndrome parameters and cathology of lymph node between healthy rhesus and rhesus infected with immunodeficiency virus. Results： The plasma viral load of rhesus in each group has reached a peak in 10-14 days after infection, and platform stage was differentiation. Compared with healthy rhesus, WBC of RP rhesus was increased significantly, while the ration of CD4 and LYM was decreased significantly. The amount of LYM of LTNP rhesus was on the high side. Compared with the other kind of rhesus, 13 2-MG and T3 of RP rhesus were reduced significantly. Lymph nodes of RP rhesus were reduced, and germinal center reduced and degenerated in platform stage. Conclusion： The difference among the 3 progress types of LTNP, NP and RP is significant in platform stage, and there is significant both spleen and kidney deficiency syndrome in RP rhesus, and it shows that the key factor for HIV/AIDS is both spleen and kidney deficiency.

关 键 词：快速进展型 一般进展型 长期不进展型 猴免疫缺陷病毒 脾肾俱虚 

分 类 号：R259[医药卫生—中西医结合] R-332[医药卫生—中医内科学]